7.5% NaHCO3 Solution
PB180421
$ 10.00
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Product Introduction
Background
Sodium bicarbonate (NaHCO₃), as a buffer, is often used in cell culture to maintain pH of medium under 4-10% CO₂. Sodium bicarbonate can provide nutrition in addition to maintaining the ph of the medium.
Ingredient Statement
With
• Ultrapure water Dissolvent
• 75 g/L NaHCO₃
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 7.5% |
| Volume | 100mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 8.2-8.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 18 months |
| Storage Condition | This product can be stored at 2-8℃ for 18 months. |
| Shipping Condition | Room Temperature |
FAQs
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Q:1Do high or low levels of sodium bicarbonate affect the pH of my tissue culture media?
AnswerSodium bicarbonate is necessary to control the pH of the solution. To maintain physiological pH, the concentration of the sodium bicarbonate in the medium must be matched with the right level of CO2 in the atmosphere above the medium in the incubator. If the sodium bicarbonate is high and the CO2 concentration is low, the pH will become alkaline. This is something that you will see in a bottle of media when it is exposed to air for long periods or when there is a lot of head space in the bottle. The media color will be pinkish or purplish. When this media is put back into a CO2 incubator, the color/pH will change back to the normal orangeish/reddish color or physiological pH. If the sodium bicarbonate is low and the CO2 is high, the pH will become acidic. This is something you will see when you put medium into a 5% or 10% CO2 incubator.
