Serum-Free Media
Serum-free media (SFM) are a type of cell culture medium formulated without animal serum, designed to support the growth and maintenance of cells while minimizing variability and contamination risks. Our R&D team has developed SFM specifically for HEK293, CHO, hybridoma, Vero, and human mesenchymal stem cells (hMSCs), completely free from animal-derived components. These ready-to-use media ensure good cell growth, high protein expression, minimal batch variation, and stability. Produced under GMP standards and ISO 13485:2016 certified, our SFM guarantee full traceability and reliability for diverse cell culture applications.
View All Serum-Free MediaKey Features
Provides defined culture conditions
Improves the stability and experimental results
Increases cell growth or productivity
Avoids the influence of serum
Simplifies purification and downstream processing
Pricella® HEK293 Serum-Free Media are designed to support high-density growth of HEK293 cells, enabling efficient transfection and protein expression, making it ideal for applications in recombinant protein production, antibody development, vaccine manufacturing, and related fields.
| Product Name | Cat. No. | Size |
|---|---|---|
| HEK293 Serum-Free Basal Medium | SF1001 | 500 mL |
| HEK293 Serum-Free Feed A | SF1002 | 50 mL |
| HEK293 Serum-Free Feed B | SF1003 | 5 mL |
CHO Serum-Free Basal Medium is fully chemically defined, free of hydrolysates, proteins, growth factors, and any animal-derived components. It is suitable for high-density suspension cultures of various Chinese Hamster Ovary (CHO) cell subtypes, including CHO-K1, CHO DG44, and CHO-S cells, enabling high-level expression of recombinant proteins and antibodies. When combined with the CHO supplements, Pricella® CHO Serum-Free Feed A and B, it supports high-density cell growth and viability, achieving even higher levels of protein/antibody expression and quality.
| Product Name | Cat. No. | Size |
|---|---|---|
| CHO Serum-Free Basal Medium | SF2001 | 500 mL |
| CHO Serum-Free Feed A | SF2002 | 100 mL |
| CHO Serum-Free Feed B | SF2003 | 10 mL |
During the process of cell passage, the growth rate of cells cultured in Pricella® CHO Serum-Free Basal Medium (Cat. No.: SF2001) is comparable to that of products from other companies.
Pricella® Hybridoma Serum-Free Medium is serum-free and contains low concentrations of protein, specifically developed for high-density growth of hybridoma cells. It is suitable for high-density suspension cultures of various hybridoma cells and for protein and antibody expression.
| Product Name | Cat. No. | Size |
|---|---|---|
| Hybridoma Serum-Free Medium | SF3001 | 500 mL |
After a short-term adaptation period using Pricella® Hybridoma Serum-Free Medium (Cat. No.: SF3001), hybridoma cells maintain stable proliferation and viability.
Hybridoma cells cultured in batch mode show relatively better growth when using Pricella® Hybridoma Serum-Free Medium (Cat. No.: SF3001) compared to products from other companies.
In different antibody expression projects, Pricella® Hybridoma Serum-Free Medium (Cat. No.: SF3001) have shown to achieve higher and more stable expression levels compared to products from other companies.
Pricella® hMSCs Serum-Free Medium is specifically formulated for the growth and expansion of human mesenchymal stem cells (hMSCs). Compared to traditional serum-supplemented media (DMEM + 10% FBS), Pricella® hMSCs Serum-Free Medium better promotes hMSCs growth and enhances consistency. Using this SFM, hMSCs can be expanded for over five passages while retaining their mesodermal differentiation potential across three lineages- osteocytes, chondrocytes, and adipocytes. Each batch of hMSCs Serum-Free Medium is validated using hMSCs to ensure consistent performance, and hMSCs cultured in serum-supplemented media can transition directly to Pricella® hMSCs Serum-Free Medium with little to no adaptation required. Additionally, this SFM can also be used for the direct expansion of hMSCs from primary human bone marrow.
| Product Name | Cat. No. | Size |
|---|---|---|
| Human Mesenchymal Stem Cells (hMSCs) Serum-Free Medium | CM-SC01 | 500 mL |
| Human Mesenchymal Stem Cells Serum-Free Medium, with Serum Replacement | CM-SC02 | 500 mL |
hMSCs were cultured and expanded using Pricella® hMSCs Serum-Free Medium (Cat. No.: CM-SC01).Flow cytometry was used to assess the expression of cell surface antigens. The results showed negative expression for CD19 (0.35%), CD34 (1.23%), and CD45 (1.08%), while positive expression was observed for CD90 (99.94%) and CD105 (97.02%).
Serum-Free Adaptation Process
Step 1: Seed cells in the original medium (serum-supplemented medium) and, once they enter the exponential growth phase, initiate serum-free adaptation.
Step 2: Culture cells in a medium composed of 75% original medium and 25% SFM.
Step 3: Culture cells in a medium composed of 50% original medium and 50% SFM.
Step 4: Culture cells in a medium composed of 25% original medium and 75% SFM.
Step 5: Culture cells in 100% SFM.
Step 1: Seed cells in the original medium (serum-supplemented medium) and, once they enter the exponential growth phase, initiate serum-free adaptation.
Step 2: Culture cells in a medium composed of 75% original medium and 25% SFM.
Step 3: Culture cells in a medium composed of 50% original medium and 50% SFM.
Step 4: Culture cells in a medium composed of 25% original medium and 75% SFM.
Step 5: Culture cells in 100% SFM.
Tips: If cells cannot adapt smoothly from 75% to 100% SFM, consider adding a step with 10% original medium and 90% SFM. For each step, it is recommended to maintain the respective ratios and passage cells for 2-3 generations. If adaptation is successful, the number of passages at each step may be reduced based on actual conditions.
