Blasticidin S (10mg/mL)

Product Introduction

Background

Blasticidin S is a kind of peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in bacteria and eukaryotes, while also active against fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is useful as a selective agent for mammalian cells and bacterial cells.
Blasticidin S is commonly used to select mammalian cell lines that have been stably transfected with the bsr/BSD/bls gene, often labeled as bsrr/bsdr/Blastr plasmid. It has a rapid and potent mode of action, and even very low antibiotic concentrations can quickly kill cells that do not carry the resistance gene.

Ingredient Statement

Matters Need Attention

1. Blasticidin S is a toxic compound, please ensure proper protection during operation.
2. This product has been filtered and sterilized by 0.1 μm filter, can be used directly after melting.
3. When using this product, attention should be paid to aseptic operation to avoid pollution.
4. The product should be thawed in 2-8℃, shake well after use, avoiding repeated freezing and thawing.
5. If there are precipitates after thawing, they can be vortexed and mixed evenly or blown with a pipette. After standing at room temperature for about 1 hour or at 37℃ in an incubator for 20-30 minutes, observe whether the precipitates can be dissolved normally, and if they can be dissolved, it can be used normally.
6. This product is a concentrated liquid, please dilute it as needed.
7. It is recommended to use the regular at 2-8℃ for preservation within one month. 6.It needs to be frozen at -5~-20℃ when not in use for a long time, and it is not suitable for long-term storage at room temperature or 2-8℃. To avoid repeated freezing and thawing, it is recommended to store it in small quantities after subpackaging.
8. This product is only for scientific research or further research use, not for diagnosis and treatment.