Dexamethasone Solution (1 M)

Product Introduction

Background

Dexamethasone (DEX) is a highly potent synthetic glucocorticoid receptor agonist with extensive biological functions. As both an apoptosis inducer and a commonly used inducer in experimental animal disease models, DEX can be used to construct a variety of disease models such as muscular dystrophy, hypertension and depression. Its mechanism of action includes inhibiting the production of inflammatory miRNA-155 exosomes in macrophages and significantly reducing the expression of inflammatory factors in neutrophils and monocytes. In addition, DEX acts as an interleukin receptor modulator and exhibits potent anti-inflammatory, antitoxin, anti-allergic and immunosuppressive effects.
In the field of organoid culture, dexamethasone is often used in combination with other key factors (e.g., hepatocyte growth factor HGF and epidermal growth factor EGF) to optimize culture conditions by regulating multiple transcription factors. For example, in liver organoid culture, the combination of DEX with HGF and EGF not only promoted the separation of hepatocytes and bile duct lineages, but also induced the formation of bile duct epithelium on the surface of the organoids, which significantly improved the functionality and structural integrity of the organoids.

Ingredient Statement

Matters Need Attention

1. This product was sterilized by 0.1 μm filtration and can be used directly after melting.
2. It is necessary to pay attention to the aseptic operation and avoid the contamination.
3. Before using, the product should be thawed at 2-8℃ and shaken thoroughly

repeated freeze-thaw cycles are not advised.
4. If precipitation happens after thawing, the contents can be resuspended by pipetting or vortex mixing. After incubating the solution at 37℃ for 20 to 30 minutes or letting it stand at room temperature for about an hour, check to see if the precipitate dissolves as intended. If the product dissolves completely, it can be used as usual.
5. This product is a concentrated solution and should be diluted prior to use as required.
6. The product should be used within a month if stored regularly at 2-8℃. Keep in a frozen state at -5~-20℃ for extended storage. Long-term storage at room temperature or between 2-8℃ is not recommended. When lesser amounts are required, aliquoting is advised to prevent repeated freeze-thaw cycles.
7. This product is for research use only.
8. Avoiding skin and eye contact. Don’t inhale dust, and take relevant precautions when operating.