PM150312
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Product Introduction
Background
The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3151 mg/L D-Glucose
• 2.5 mM L-Glutamine
• 2438 mg/L NaHCO₃
• 8.1 mg/L Phenol red
• 0.5 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: BMC CANCER (2025) IF: 3.4
DOI: 10.1186/s12885-025-13428-1
Product Cited: Leibovitz's L-15 Medium, DMEM/F12 Medium
Journal: Breast Cancer-Targets and Therapy (2025) IF: 3.3
DOI: 10.2147/BCTT.S506783
Product Cited: MCF 10A Cells Line, DMEM/F12 Medium, Leibovitz's L-15 Medium, MDA-MB-231 Cells Line
Journal: EXPERIMENTAL CELL RESEARCH (2025) IF: 3.3
DOI: 10.1016/j.yexcr.2025.114583
Product Cited: DMEM/F12 Medium, HK-2 Cells Line
Journal: JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2025) IF: 3.2
DOI: 10.1002/jbt.70291
Product Cited: DMEM/F12 Medium, HK-2 Cells Line
Journal: BIOLOGY OF REPRODUCTION (2025) IF: 3.1
Product Cited: DMEM/F12 Medium, M199 Medium