PM150312
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Product Introduction
Background
The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3151 mg/L D-Glucose
• 2.5 mM L-Glutamine
• 2438 mg/L NaHCO₃
• 8.1 mg/L Phenol red
• 0.5 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: Open Medicine (2021) IF: 2.100
Product Cited: U-2 OS [U2OS] Cells Line, HOS Cells Line, Saos-2 Cells Line, Fetal Bovine Serum, DMEM/F12, hFOB 1.19 Cells Line
Journal: Oxidative Medicine and Cellular Longevity (2021) IF:
DOI: 10.1155/2021/5710440
Product Cited: HK-2 Cells Line, DMEM/F12, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×
Journal: Cancer Letters (2020) IF: 9.700
DOI: 10.1016/j.canlet.2020.06.025
Product Cited: DMEM/F12
Journal: EXPERT OPINION ON THERAPEUTIC TARGETS (2020) IF: 5.473
DOI: 10.1080/14728222.2020.1737013
Product Cited: DMEM/F12 Medium
Journal: OncoTargets and Therapy (2020) IF: 4.000
DOI: 10.2147/OTT.S281962
Product Cited: Leibovitz's L-15 Medium, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100 ×, MEM Medium, DMEM/F12 Medium, McCoy's 5A Medium, Leibovitz's L-15, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×, MEM(with NEAA), 10 mg/mL Recombinant Human Insulin Solution, McCoy's 5A, DMEM/F12