DMEM/F12, with HEPES

Cat. No.: PM150310 Publications(30) Manual
DMEM/F12, with HEPES - 1
  • DMEM/F12, with HEPESDMEM/F12, with HEPES - 1

PM150310

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500mL

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Product Introduction

Background

The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Ingredient Statement
With

• 3151 mg/L D-Glucose

• 15 mM HEPES

• 2.5 mM L-Glutamine

• 1200 mg/L NaHCO₃

• 8.1 mg/L Phenol red

• 0.5 mM Sodium pyruvate

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

Specifications
Concentration
Volume 500mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.2-7.4
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Documents

Publications

Absence of MerTK disrupts spermatogenesis in an age-dependent manner

Journal: MOLECULAR AND CELLULAR ENDOCRINOLOGY (2022) IF: 4.369

DOI: 10.1016/j.mce.2022.111815

Product Cited: DMEM/F12, with HEPES Medium

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