PM150910
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Product Introduction
Background
The Ham's F-12K medium increases the concentration of amino acids and pyruvic acid on the basis of Ham's F-12, reduces the concentration of glucose, and modifies the composition and content of the salt.The Ham's F-12K medium was initially designed to culture primary human hepatocytes and differentiated cells of rats and chickens.
The Ham's F-12K medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1260 mg/L D-Glucose
• 2 mM L-Glutamine
• 2500 mg/L NaHCO₃
• 3 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: International Journal Of Biological Macromolecules (2020) IF: 8.200
DOI: 10.1016/j.ijbiomac.2020.03.060
Product Cited: Ham's F-12K, A549 [A-549] Cells Line, A549/Tax Cells Line
Journal: Acta Physiologica (2020) IF: 5.542
DOI: 10.1111/apha.13555
Product Cited: Ham's F-12K Medium
Journal: Bioengineered (2020) IF: 4.900
DOI: 10.1080/21655979.2020.1860492
Product Cited: Ham's F-12K, A549 [A-549] Cells Line, SK-MES-1 Cells Line, NCI-H1437 Cells Line, NCI-H1975 Cells Line
Journal: Bioengineered (2020) IF: 4.900
DOI: 10.1080/21655979.2020.1757224
Product Cited: SW620 Cells Line, SW480 [SW-480] Cells Line, Caco-2 Cells Line, LoVo Cells Line, 293T [HEK-293T] Cells Line, Ham's F-12K
Journal: Cellular Signalling (2020) IF: 4.800
DOI: 10.1016/j.cellsig.2020.109707
Product Cited: HCT 116 Cells Line, LoVo Cells Line, HT-29 Cells Line, Ham's F-12K