Mouse Brain Microvascular Endothelial Cell Isolation and Culture Kit

Cat. No.: P-CA-701

P-CA-701

$ 680.00

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3Tests 10Tests

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Product Introduction Documents

Product Introduction

Background

The Mouse Brain Microvascular Endothelial Cells Isolation and Culture Kit is specifically developed for the extraction of primary Mouse brain microvascular endothelial cells. As validated, standard operation using this kit enables the acquisition of one flask of cells (T-25 culture flask) per 1 Test, with a cell count exceeding 1×10⁶ cells. When passaged at a 1:2 ratio, the cells can undergo 1-2 passages. Through immunofluorescence analysis, the cell purity (CD31-positive rate) has been confirmed to exceed 90%.

Specifications

Volume	3Tests/10Tests
Shipping Condition	Ice bag

Matters Need Attention

1. Prior to formal experiments, it is recommended to conduct anatomical simulation training using 1-2 normal rats to familiarize operators with procedural workflows and improve tissue dissociation efficiency.
2. During the entire tissue dissociation process, place the small dish containing the tissue on an ice tray/ice box (2-8℃) to maintain hypothermic conditions. Critical precautions: Monitor temperature rigorously to prevent ice crystal formation in tissues/liquids.
3. Reagent preparation or dispensing must strictly adhere to aseptic technique protocols. After dispensing, seal the containers immediately with a sealing film, use them promptly to avoid repeated freeze-thaw cycles or contamination.

Kit Components

Name	Size	Appearance	Storage and Expiration Dat
Specialized Washing Solution For Mouse Microvascular Endothelial Cells	3Tests/10Tests	250 mL/500 mL×2	2-8℃, 1 year
Specialized Digestive Solution A For Mouse Brain Microvascular Endothelial Cells	3Tests/10Tests	15 mL/50 mL	-5~-20℃, 1 year
Specialized Digestive Solution B For Mouse Brain Microvascular Endothelial Cells	3Tests/10Tests	1.8 mL/6 mL	-5~-20℃, 1 year
Specialized Isolation Solution For Mouse Brain Microvascular Endothelial Cells	3Tests/10Tests	15 mL/50 mL	2-8℃, 1 year
Basic Culture Medium For Mouse Brain Microvascular Endothelial Cells	3Tests/10Tests	50 mL/100 mL	2-8℃, 1 year
Supplement For Mouse Brain Microvascular Endothelial Cells	3Tests/10Tests	5 mL/10 mL	-5~-20℃, 1 year
Planting Solution For Mouse Brain Microvascular Endothelial Cells	3Tests/10Tests	3 mL/10 mL	-5~-20℃, 1 year
Screening Solution For Mouse Brain Microvascular Endothelial Cells	3Tests/10Tests	45 μL/150 μL	-5~-20℃, 1 year
100 μm Cell Filter	3Tests/10Tests	3 pcs/10 pcs	Room temperature, 3 years

Documents

Manual

MSDS