PM150710
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Product Introduction
Background
McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3000 mg/L D-Glucose
• 1.5 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
This product is manufactured in a GMP-compliant factory, formulated with water for injection, and has obtained ISO 9001 and ISO 13485 certifications.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Medium | Components 3000 mg/L D-Glucose 1.5 mM L-Glutamine 2200 mg/L NaHCO₃ 10 mg/L Phenol Red |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Publications
Journal: ONCOGENE (2023) IF: 8.000
DOI: 10.1038/s41388-023-02656-z
Product Cited: McCoy's 5A Medium
Journal: ONCOGENE (2023) IF: 8.0
DOI: 10.1038/s41388-023-02879-0
Product Cited: A549 [A-549] Cells Line, NCI-H1299 Cells Line, 786-O [786-0] Cells Line, OS-RC-2 Cells Line, 293T [HEK-293T] Cells Line, BEAS-2B Cells Line, McCoy's 5A
Journal: eLife (2023) IF: 7.7
DOI: 10.7554/eLife.85412
Product Cited: U-2 OS [U2OS] Cells Line, McCoy's 5A Medium
Journal: Journal of Translational Medicine (2023) IF: 7.400
DOI: 10.1186/s12967-022-03862-1
Product Cited: AML12 Cells Line, McCoy's 5A
Journal: ANALYTICAL CHEMISTRY (2023) IF: 7.4
DOI: 10.1021/acs.analchem.3c02333
Product Cited: SK-BR-3 [SKBR3] Cells Line, MCF7 [MCF-7] Cells Line, MDA-MB-231 Cells Line, McCoy's 5A
