PM150710
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Product Introduction
Background
McCoy's 5A medium was initially used specifically for the culture of Novikoff Hepatoma cells. Unlike other medium, McCoy's 5A medium contains reduced glutathione, bacterial peptone and high concentrations of glucose. McCoy's 5A is widely used in the culture of primary cells, such as bone marrow, skin, gingiva, kidney, spleen, lung, rat embryo, omentum and so on. In addition, McCoy's 5A medium is also used for tissue biopsy culture, the establishment of cell line, and the culture of some lymphocytes and more difficult cells.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3000 mg/L D-Glucose
• 1.5 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: Pharmaceutics (2023) IF: 5.4
DOI: 10.3390/pharmaceutics15072012
Product Cited: HT-29 Cells Line, Hep G2 Cells Line, PC-3 Cells Line, McCoy's 5A, MEM(with NEAA), Ham's F-12K
Journal: Aging-US (2023) IF: 5.2
Product Cited: HT-1080 [HT1080] Cells Line, McCoy's 5A Medium, MEM Medium, A-204 [A204] Cells Line
Journal: BIOORGANIC CHEMISTRY (2023) IF: 5.100
DOI: 10.1016/j.bioorg.2023.106506
Product Cited: McCoy's 5A
Journal: INFLAMMATION (2023) IF: 5.1
DOI: 10.1007/s10753-023-01909-z
Product Cited: McCoy's 5A
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH (2023) IF: 5.1
DOI: 10.1016/j.bbamcr.2023.119553
Product Cited: HOS Cells Line, Saos-2 Cells Line, SW 1353 Cells Line, McCoy's 5A