Mouse Cardiac Fibroblast Isolation and Culture Kit
P-CA-716
$ 480.00
Select a size
Quantity
In stock
Add to CartFor order requirements and technical needs, please Contact Us
Product Introduction
Background
This kit is specially developed for extracting primary mouse cardiac fibroblasts. It uses low-temperature digestion and differential adhesion methods to isolate and purify mouse cardiac fibroblasts. After laboratory verification, it can isolate more than 1 × 10⁶ target cells in each experiment (1 Test). Immunofluorescence identification (Vimentin) results show that the cell purity is above 90%.
Specifications
| Volume | 3Tests/10Tests |
| Valid Period | 12 months |
| Shipping Condition | Ice bag |
Matters Need Attention
1. Before the formal experiment, it is recommended to use 1-2 normal mice for simulated anatomy to familiarize the operation process and improve the speed of tissue separation.
2. During the entire anatomical sampling, it is recommended to place the culture dish containing the tissue on an ice plate (2-8℃) to maintain low temperature, but be careful not to freeze the tissue and liquid due to low temperature.
3. The culture medium contains nutrients necessary for microbial growth. Please open it in a super clean bench, divide it according to the required amount, and seal the bottle mouth with a sealing film for immediate use to avoid contamination.
Kit Components
| Name | Size | Appearance | Storage and Expiration Dat |
| Special Washing Solution For Mouse Cardiac Fibroblasts | 3Tests/10Tests | 250 mL/500 mL | 2-8℃, 1 year |
| Specialized Digestive Solution For Mouse Cardiac Fibroblasts | 3Tests/10Tests | 50 mL/150 mL | -5~-20℃, 1 year |
| Digestive Termination Fluid For Mouse Cardiac Fibroblasts | 3Tests/10Tests | 10 mL/30 mL | -5~-20℃, 1 year |
| Basic Culture Medium For Mouse Cardiac Fibroblasts | 3Tests/10Tests | 100 mL/250 mL | 2-8℃, 1 year |
| Supplement For Mouse Cardiac Fibroblasts | 3Tests/10Tests | 10 mL/25 mL | -5~-20℃, 1 year |
| 100 μm Cell Filter | 3Tests/10Tests | 3 pcs/10 pcs | Room temperature, 3 years |
| 70 μm Cell Filter | 3Tests/10Tests | 3 pcs/10 pcs | Room temperature, 3 years |
