Mouse Cardiac Microvascular Endothelial Cell Isolation and Culture Kit
P-CA-714
$ 680.00
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Product Introduction
Background
This kit is developed specifically for the isolation of various primary mouse cardiac microvascular endothelial cells. The tissue is digested using a two-step digestion method, and the cells are purified by density gradient centrifugation. After laboratory verification, it can isolate more than 1×10⁶ cells, and the immunofluorescence identification (CD31 positive rate) more than 90%
Specifications
| Volume | 3Tests/10Tests |
| Valid Period | 12 months |
| Shipping Condition | Ice bag |
Matters Need Attention
1. Before the formal experiment, it is recommended to use 1-2 normal mice for simulated anatomy to familiarize the operation process and improve the speed of tissue separation.
2. During the entire anatomical sampling, it is recommended to place the culture dish containing the tissue on an ice plate (2-8℃) to maintain low temperature, but be careful not to freeze the tissue and liquid due to low temperature.
3. The culture medium contains nutrients necessary for microbial growth. Please open it in a super clean workbench, divide it according to the required amount, and seal the bottle mouth with a sealing film for immediate use to avoid contamination.
4. All separation solutions in this kit may experience the precipitation of crystal particles at the bottle mouth and turbidity of the solution during storage, which is a normal phenomenon and can be used with no problem.
Kit Components
| Name | Size | Appearance | Storage and Expiration Dat |
| Specialized Washing Solution For Mouse Cardiac Microvascular Endothelial Cells | 3Tests/10Tests | 250 mL/500 mL | 2-8℃, 1 year |
| Digestive Solution A For Mouse Cardiac Microvascular Endothelial Cells | 3Tests/10Tests | 15 mL/50 mL | -5~-20℃, 1 year |
| Digestive Solution B For Mouse Cardiac Microvascular Endothelial Cells | 3Tests/10Tests | 3 mL/10 mL | -5~-20℃, 1 year |
| Basic Culture Medium For Mouset Cardiac Microvascular Endothelial Cells | 3Tests/10Tests | 100 mL/250 mL | 2-8℃, 1 year |
| Supplement For Mouse Cardiac Microvascular Endothelial Cells | 3Tests/10Tests | 10 mL/25 mL | -5~-20℃, 1 year |
| Specialized Isolation Solution A For Mouse Cardiac Microvascular Endothelial Cells | 3Tests/10Tests | 10 mL/30 mL | 2-8℃, 1 year |
| Specialized Isolation Solution B For Mouse Cardiac Microvascular Endothelial Cells | 3Tests/10Tests | 15 mL/50 mL | 2-8℃, 1 year |
| Planting Solution For Mouse Cardiac Microvascular Endothelial Cells | 3Tests/10Tests | 3 mL/10 mL | -5~-20℃, 1 year |
| 70 μm Cell Filter | 3Tests/10Tests | 3 pcs/10 pcs | Room temperature, 3 years |
