PM150410
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Product Introduction
Background
MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• NEAA
• Earle's Balanced salt
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: ADVANCED FUNCTIONAL MATERIALS (2025) IF: 18.5
Product Cited: MEM, with NEAA Medium, Caco-2 Cells Line
Journal: Acta Pharmaceutica Sinica B (2025) IF: 14.7
DOI: 10.1016/j.apsb.2025.02.037
Product Cited: Penicillin-Streptomycin Solution, 100 ×, MEM, with NEAA Medium, DMEM (High glucose) Medium, Phosphate Buffer (PBS, 1 ×), Fetal Bovine Serum
Journal: Nature Communications (2025) IF: 14.7
DOI: 10.1038/s41467-025-59042-4
Product Cited: MEM, with NEAA Medium, SH-SY5Y [SHSY-5Y] Cells Line, HMC3 Cells Line
Journal: Advanced Science (2025) IF: 14.3
Product Cited: MEM, with NEAA Medium, Human Lung Fibroblast Cells, HMC3 Cells Line
Journal: Natural Product Communications (2025) IF: 1.5
DOI: 10.1177/1934578X241306938
Product Cited: Hep G2 Cells Line, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100 ×, Phosphate Buffer (PBS, 1 ×), MEM, with NEAA Medium