PM150410
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Product Introduction
Background
MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• NEAA
• Earle's Balanced salt
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Publications
Journal: BMC Urology (2024) IF: 1.7
DOI: 10.1186/s12894-024-01666-7
Product Cited: DU 145 Cells Line, Ham's F-12K Medium, PC-3 Cells Line, MEM, with NEAA Medium
Journal: JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH (2024) IF: 1.3
DOI: 10.1080/10286020.2024.2375291
Product Cited: MEM, with NEAA Medium, Fetal Bovine Serum, Hep G2 Cells Line, Dulbecco's Phosphate Buffer (DPBS, 1 ×), without calcium, magnesium, phenol red
Journal: SCIENCE OF THE TOTAL ENVIRONMENT (2023) IF: 9.8
DOI: 10.1016/j.scitotenv.2023.166057
Product Cited: Caco-2 Cells Line, MEM(with NEAA), 200 mM L-Glutamine Solution, 100×
Journal: CANCER LETTERS (2023) IF: 9.700
DOI: 10.1016/j.canlet.2023.216058
Product Cited: SV-HUC-1 Cells Line, UM-UC-3 Cells Line, J82 Cells Line, 5637 Cells Line, SW 780 [SW-780, SW780] Cells Line, T24 [T-24] Cells Line, TCCSUP Cells Line, Ham's F-12K, MEM(with NEAA), Leibovitz's L-15, McCoy's 5A
Journal: ENVIRONMENTAL POLLUTION (2023) IF: 8.900
DOI: 10.1016/j.envpol.2023.121729
Product Cited: MEM(with NEAA), Fetal Bovine Serum
