PM150410
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Product Introduction
Background
MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• NEAA
• Earle's Balanced salt
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
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Publications
Journal: IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2023) IF: 3.300
DOI: 10.1080/08923973.2023.2165943
Product Cited: MCF 10A Cells Line, Leibovitz's L-15, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×, MEM(with NEAA), MCF 10A Cells Complete Medium
Journal: Immunity Inflammation and Disease (2023) IF: 3.2
DOI: 10.1002/iid3.1071
Product Cited: Hep G2 Cells Line, MEM(with NEAA), Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×
Journal: Journal of Diabetes Investigation (2023) IF: 3.2
DOI: 10.1111/jdi.14107
Product Cited: HK-2 Cells Line, MEM(with NEAA)
Journal: JOURNAL OF MOLECULAR HISTOLOGY (2023) IF: 3.2
DOI: 10.1007/s10735-023-10156-3
Product Cited: DU 145 Cells Line, PC-3 Cells Line, Ham's F-12K, MEM(with NEAA), Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×
Journal: Journal of Diabetes Investigation (2023) IF: 3.2
DOI: 10.1111/jdi.14050
Product Cited: Human Adipose-derived Mesenchymal Stem cells, Human Adipose-derived Mesenchymal Stem cells Complete Medium, Human Adipocyte cells, Human Adipocyte cells Complete Medium, Hep G2 Cells Line, MEM(with NEAA)
