PM150410
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Product Introduction
Background
MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• NEAA
• Earle's Balanced salt
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: Neural Regeneration Research (2022) IF: 6.100
Product Cited: HMC3 Cells Line, MEM(with NEAA), Fetal Bovine Serum, DMEM(Low glucose), without phenol red
Journal: Frontiers in Pharmacology (2022) IF: 5.988
DOI: 10.3389/fphar.2022.1088294
Product Cited: Penicillin-Streptomycin Solution, 100 ×, MEM, with NEAA Medium, NCTC clone 929 [L cell, L-929] Cells Line, Fetal Bovine Serum
Journal: INTERNATIONAL JOURNAL OF ONCOLOGY (2022) IF: 5.884
Product Cited: MIA PaCa-2 Cells Line, MEM, with NEAA Medium, PANC-1 Cells Line, IMDM Medium, Donor Equine Serum, HPAF-II Cells Line, CFPAC-1 Cells Line
Journal: FASEB JOURNAL (2022) IF: 5.834
Product Cited: MEM, with NEAA Medium
Journal: Biosensors-Basel (2022) IF: 5.743
DOI: 10.3390/bios12020089
Product Cited: MEM, with NEAA Medium, HMC3 Cells Line