MEM, with NEAA

Cat. No.: PM150410 Publications(278) Manual
MEM, with NEAA - 1
  • MEM, with NEAAMEM, with NEAA - 1

PM150410

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500mL

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Product Introduction

Background

MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Ingredient Statement
With

• 1000 mg/L D-Glucose

• 2 mM L-Glutamine

• 2200 mg/L NaHCO₃

• 10 mg/L Phenol red

• NEAA

• Earle's Balanced salt

Without

• HEPES

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

Specifications
Concentration
Volume 500mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.2-7.4
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Documents

Publications

Thorium inhibits human respiratory chain complex IV (cytochrome c oxidase)

Journal: Journal of Hazardous Materials (2022) IF: 14.200

DOI: 10.1016/j.jhazmat.2021.127546

Product Cited: MG-63 Cells Line, Hep G2 Cells Line, MEM(with NEAA), DMEM/F12, Fetal Bovine Serum

YTHDC1 is downregulated by the YY1/HDAC2 complex and controls the sensitivity of ccRCC to sunitinib by targeting the ANXA1-MAPK pathway

Journal: JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH (2022) IF: 12.700

DOI: 10.1186/s13046-022-02460-9

Product Cited: 786-O [786-0] Cells Line, A-498 Cells Line, STR Identification, MEM(with NEAA)

New injectable chitosan-hyaluronic acid based hydrogels for hemostasis and wound healing

Journal: CARBOHYDRATE POLYMERS (2022) IF: 11.200

DOI: 10.1016/j.carbpol.2022.119767

Product Cited: NCTC clone 929 [L cell, L-929] Cells Line, MEM(with NEAA)

FGF21 promotes migration and differentiation of epidermal cells during wound healing via SIRT1 dependent autophagy

Journal: British Journal Of Pharmacology (2021) IF: 9.500

DOI: 10.1111/bph.15701

Product Cited: Hacat Cells Line, MEM(with NEAA)

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