PM150410
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Product Introduction
Background
MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• NEAA
• Earle's Balanced salt
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: Signal Transduction and Targeted Therapy (2025) IF: 40.8
DOI: 10.1038/s41392-025-02245-4
Product Cited: HL-1 Cells Line, Tyrode's Solution, without calcium, Penicillin-Streptomycin Solution, 100 ×, DMEM/F12 Complete Medium (10), 4T1 Cells Line, MEM, with NEAA Medium
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY (2025) IF: 4.8
DOI: 10.1016/j.intimp.2025.114884
Product Cited: MEM, with NEAA Medium
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY (2025) IF: 4.8
DOI: 10.1016/j.intimp.2025.114675
Product Cited: Hep G2 Cells Line, MEM, with NEAA Medium
Journal: MOLECULAR NEUROBIOLOGY (2025) IF: 4.6
DOI: 10.1007/s12035-025-04726-7
Product Cited: Ham's F-12 Medium, MEM, with NEAA Medium, SH-SY5Y [SHSY-5Y] Cells Line, DMEM (High glucose) Medium, 0.25% Trypsin Solution, Penicillin-Streptomycin Solution, 100 ×
Journal: MOLECULAR PHARMACEUTICS (2025) IF: 4.5
DOI: 10.1021/acs.molpharmaceut.4c01175
Product Cited: MEM Medium, MEM, with NEAA Medium, C6 Cells Line, U-87 MG Cells Line