PM150410
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Product Introduction
Background
MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• NEAA
• Earle's Balanced salt
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Publications
Journal: Frontiers in Medicine (2025) IF: 3.1
DOI: 10.3389/fmed.2024.1485224
Product Cited: MEM, with NEAA Medium, Hacat Cells Line
Journal: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2025) IF: 3.1
DOI: 10.1007/s12010-024-05161-5
Product Cited: Hep G2 Cells Line, HuH-7 Cells Line, DMEM (High glucose) Medium, MEM, with NEAA Medium
Journal: MOLECULAR CARCINOGENESIS (2025) IF: 3.0
DOI: 10.1002/mc.23895
Product Cited: A172 Cells Line, LN229 [LN-229; LNT-229] Cells Line, T98G Cells Line, U-87 MG Cells Line, MEM, with NEAA Medium, Penicillin-Streptomycin Solution, 100 ×, U251 Cells Line
Journal: MOLECULAR CARCINOGENESIS (2025) IF: 3.0
DOI: 10.1002/mc.23912
Product Cited: MEM, with NEAA Medium, MCF7 [MCF-7] Cells Line, ZR-75-1 Cells Line, Penicillin-Streptomycin Solution, 100 ×, Donor Equine Serum, DMEM/F12 Medium, MCF 10A Cells Line, MDA-MB-453 Cells Line, 200 mM L-Glutamine Solution, 100 ×, 10 mg/mL Recombinant Human Insulin Solution, BT-474 [BT474] Cells Line, MEM Non-Essential Amino Acid Solution (NEAA), 100 ×
Journal: EUROPEAN JOURNAL OF MEDICAL RESEARCH (2025) IF: 2.8
DOI: 10.1186/s40001-025-02676-3
Product Cited: MEM, with NEAA Medium, Penicillin-Streptomycin Solution, 100 ×
