Mergene1000^® NIH/3T3 Cell-Specific DNA Transfection Reagent

Product Introduction

Background

Mergene1000^® NIH/3T3 Cell-Specific DNA Transfection Reagent is a high-performance DNA transfection reagent designed for the delivery of plasmid DNA. It is characterized by its strong DNA transfection capability and is specifically formulated for use with NIH/3T3 cells, achieving high transfection efficiency. The reagent is distinguished by its low toxicity, excellent stability, ease of operation, and high reproducibility.

Matters Need Attention

1. The cell inoculation amount and transfection ratio provided above are based on experiments conducted with NIH/3T3 cells and are for reference only. The specific experimental dosage should be adjusted according to the actual conditions.
2. The product is transported with ice bag and can be aliquoted and stored upon use to avoid multiple prolonged openings of the lid.
3. DMEM (High glucose) medium should be prepared separately for the dilution of plasmid DNA and transfection reagents.
4. During transfection, ensure that the degree of cell confluence is not less than 60%, and it is generally maintained at around 60% to 70%. The specific plating density can be adjusted according to the actual conditions of the cells.
5. After transfection, there is no need to remove the transfection complex or replace with fresh culture medium. The actual operation can be based on the cell status, after transfection culture 4-6 hours to choose to change the medium.
6. The use of high purity endotoxin-free DNA is helpful to obtain higher transfection efficiency.
7. The plasmid concentration and reagent amount should be optimized for the first use to obtain the highest transfection efficiency.
8. For research use only.
9. For your safety and health, please wear experimental clothes and wear disposable gloves aseptic operation.