Mouse Liver Sinusoidal Endothelial Cell Isolation and Culture Kit

Cat. No.: P-CA-709

P-CA-709

$ 580.00

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Product Introduction Documents

Product Introduction

Background

The Mouse liver Sinusoidal Endothelial Cell Isolation and Culture Kit is specifically developed for extracting primary mouse liver sinusoidal endothelial cells. After validated, standard operation using this kit enables the acquisition of one flask of cells (T-25 culture flask) per 1 Test, with a cell count exceeding 1×106 cells When passaged at a 1:2 ratio, the cells can undergo 2-3 passages. Through immunofluorescence analysis, the cell purity (CD31/CD14-positive rate) has been confirmed to exceed 90%.

Specifications

Volume	3Tests/10Tests
Valid Period	12 months
Shipping Condition	Ice bag

Matters Need Attention

1. Prior to formal experiments, it is recommended to conduct anatomical simulation training using 1-2 normal mice to familiarize operators with procedural workflows and improve tissue dissociation efficiency.
2. During the entire tissue dissociation process, place the small dish containing the tissue on an ice tray/ice box (2-8℃) to maintain hypothermic conditions. Critical precautions: Monitor temperature rigorously to prevent ice crystal formation in tissues/liquids.
3. Reagent preparation or dispensing must strictly adhere to aseptic technique protocols. After dispensing, seal the containers immediately with a sealing film, use them promptly to avoid repeated freeze-thaw cycles or contamination.
4. All special separation solution in this kit may exhibit crystal precipitation at the bottle mouth and mild turbidity during storage. These phenomena are normal and do not affect the performance or usability of the reagents.

Kit Components

Name	Size	Appearance	Storage and Expiration Dat
Specialized Washing Solution For Mouse Liver Sinusoidal Endothelial Cells	3Tests/10Tests	250 mL/500 mL×2	2-8℃, 1 year
Diluent Of Digestive Enzyme For Mouse Liver Sinusoidal Endothelial Cells	3Tests/10Tests	15 mL/50 mL	2-8℃, 1 year
Specific Digestive Enzyme For Mouse Liver Sinusoidal Endothelial Cells	3Tests/10Tests	1.5 mL/5 mL	-5~-20℃, 1 year
Special Separation Solution A For Mouse Liver Sinusoidal Endothelial Cells	3Tests/10Tests	20 mL/75 mL	2-8℃, 1 year
Special Separation Solution B For Mouse Liver Sinusoidal Endothelial Cells	3Tests/10Tests	20 mL/75 mL	2-8℃, 1 year
Special Separation Solution C For Mouse Liver Sinusoidal Endothelial Cells	3Tests/10Tests	10 mL/30 mL	2-8℃, 1 year
Basic Culture Medium For Mouse Liver Sinusoidal Endothelial Cells	3Tests/10Tests	100 mL/300 mL	2-8℃, 1 year
Supplement For Mouse Liver Sinusoidal Endothelial Cells	3Tests/10Tests	10 mL/30 mL	-5~-20℃, 1 year
70 μm Cell Filter	3Tests/10Tests	3 pcs/10 pcs	Room temperature, 3 years
100 μm Cell Filter	3Tests/10Tests	3 pcs/10 pcs	Room temperature, 3 years

Documents

Manual

MSDS