Micro Exosome Isolation Kit
P-CA-501
$ 648.00
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Product Introduction
Background
This kit utilizes our proprietary homogeneous liquid magnetic beads, engineered for the selective capture of exosomes while minimizing the adsorption of contaminants. This results in the isolation of exosomes with superior purity and high recovery rates. With its user-friendly design, exceptional purity, and robust recovery efficiency, it is ideally suited for exosome isolation from complex biological matrices such as plasma and serum. The isolated exosomes are versatile for a wide range of downstream applications, including Western blot (WB) analysis, nanoparticle tracking analysis (NTA), nanoparticle flow cytometry for particle size characterization, electron microscopy, omics profiling, and functional studies in cellular and animal models.
Separation Method
PS (Phosphatidylserine)-Affinity Magnetic Beads Method
Product Features
1 .Low Starting Volume: Enables the enrichment of exosomes from limited or precious samples.
2. Rapid Processing: Exosome isolation can be completed within 2 hours.
3. Ease of Use: Requires only a standard magnetic rack; no specialized equipment is needed.
4. High Reproducibility: Minimal operational proficiency required, ensuring consistent results.
5. High Recovery Rate: Achieves a recovery rate exceeding 90%.
6. High Purity: Purified exosomes are suitable for direct RNA and protein extraction.
7. Excellent Integrity: Magnetic bead-based isolation preserves exosome integrity better than other methods.
8. High Throughput: Capable of processing multiple samples simultaneously and compatible with automated systems.
9. Wide Applicability: Suitable for isolating exosomes from complex samples like plasma and serum, as well as simpler samples such as cell culture supernatants and urine.
Specifications
| Volume | 20Tests/50Tests |
| Acteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Green_Features | Sustainable packaging |
| Valid Period | 18 months |
| Storage Condition | This product can be stored at 2-8℃ for 18 months with shading light. |
| Shipping Condition | Ice bag |
Sample Processings
1.Sample Processing
1) Cell Removal: Centrifuge the sample at 300 × g for 5 minutes at 4℃. Carefully transfer the supernatant to a new centrifuge tube.
Note: This step can be skipped for cell-free samples.
2) Removal of Cellular Debris: Centrifuge the supernatant obtained from Step 1 at 2,000 × g for 10 minutes at 4°C. Transfer the supernatant to a new centrifuge tube.3) Removal of Large Particles: Centrifuge the supernatant obtained from Step 2 at 14,000 × g for 30 minutes at 4℃. Carefully transfer the resulting supernatant to a new centrifuge tube.
2. Exosomes Enrichment
1) Magnetic Bead Preparation: Add 100 μL of Exosome Beads to a 1.5 mL centrifuge tube. Place the tube on a magnetic rack for 1 minute and discard the storage buffer.
2) Sample Incubation: Add 500 μL of the preprocessed sample to the magnetic beads (if the volume is less than 500 μL, top up with Solution A). Secure the centrifuge tube on an orbital shaker and incubate at room temperature with gentle rotation (17 rpm) for 30 minutes.
Note: During the binding process, magnetic beads may aggregate in certain samples. This will not affect the separation efficiency.
3) Separation of Beads: Place the tube on a magnetic rack and let it stand for 30 seconds. Discard the supernatant.
4) Washing: Add 1 mL of Solution A and mix by gentle inversion. Place the tube on the magnetic rack, let it stand for 30 seconds, and discard the supernatant.
5) Repeat Washing: Repeat Step 4 two more times, retaining the magnetic beads at the end.
3. Exosome Isolation
Elution: Add 100 μL of Elution Buffer to the magnetic beads containing the enriched exosomes. Mix by pipetting.
1) Incubation: Secure the centrifuge tube on an orbital shaker and incubate at room temperature with gentle rotation (17 rpm) for 30 minutes.
2) Separation of Exosomes: Place the tube on a magnetic rack and let it stand for 30 seconds. Carefully transfer the supernatant to a new 1.5 mL centrifuge tube. This supernatant contains the isolated exosomes.
Note: Exosomes should not be stored in Elution Buffer for extended periods. For long-term storage, use ultrafiltration tubes (MWCO: 10 kDa) to replace the buffer with PBS.
Kit Components
| Name | Size | Appearance | Storage and Expiration Dat |
| Exosome Beads | 20Tests/50Tests | 2 mL/5 mL | 2-8℃, shading light |
| Solution A | 20Tests/50Tests | 100 mL/125 mL×2 | 2-8℃, shading light |
| Elution Buffer | 20Tests/50Tests | 2 mL/5 mL | 2-30℃, shading light |
