Mouse Cortical Neuron Isolation and Culture Kit

Cat. No.: P-CA-704

P-CA-704

$ 518.00

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Product Introduction Documents

Product Introduction

Background

The Mouse Cortical Neuron Isolation and Culture Kit is specifically developed to extract primary Mouse Cortical Neurons. As validated, standard operation using this kit enables the acquisition of one flask of cells (T-25 culture flask) per 1 Test, with a cell count exceeding 1×10⁶ cells. Through immunofluorescence analysis, the cell purity (β-Tubulin Ⅲ-positive rate) has been confirmed to exceed 90%.

Specifications

Volume	3Tests/10Tests
Shipping Condition	Ice bag

Matters Need Attention

1. Prior to formal experiments, it is recommended to conduct anatomical simulation training using 1-2 normal mice to familiarize operators with procedural workflows and improve tissue dissociation efficiency.
2. During the entire tissue dissociation process, place the small dish containing the tissue on an ice tray/ice box (2-8℃) to maintain hypothermic conditions. Critical precautions: Monitor temperature rigorously to prevent ice crystal formation in tissues/liquids.
3. Reagent preparation or dispensing must strictly adhere to aseptic technique protocols. After dispensing, seal the containers immediately with a sealing film, and use them promptly to avoid repeated freeze-thaw cycles or contamination.
4. Cortex neuron cells are terminally differentiated cells that lack proliferative capacity and exhibit high fragility. Improper enzymatic digestion can readily induce cell injury. During cell seeding, it is recommended to calculate the seeding density based on experimental requirements and inoculate into corresponding culture vessels. The recommended conversion ratios are as follows: one T25 flask ≈ one 6 cm dish ≈ two wells of a 6-well plate ≈ five to six wells of a 12-well plate.

Kit Components

Name	Size	Appearance	Storage and Expiration Dat
Specialized Washing Solution For Mouse Cortex Neuron Cells	3Tests/10Tests	250 mL/500 mL×2	2-8℃, 1 year
Specialized Digestive Solution For Mouse Cortex Neuron Cells	3Tests/10Tests	24 mL/80 mL	-5~-20℃, 1 year
Basic Culture Medium For Mouse Cortex Neuron Cells	3Tests/10Tests	50 mL/100 mL	2-8℃, 1 year
Supplement For Mouse Cortex Neuron Cells	3Tests/10Tests	2.5 mL/5 mL	-5~-20℃, 1 year
Digestion Termination Solution For Mouse Cortex Neuron Cells	3Tests/10Tests	75 mL/250 mL	2-8℃, 1 year
Planting Solution For Mouse Cortex Neuron Cells	3Tests/10Tests	3 mL/10 mL	-5~-20℃, 1 year
Screening Fluid For Mouse Cortex Neuron Cells	3Tests/10Tests	45 μL/150 μL	-5~-20℃, 1 year
70 μm Cell Filter	3Tests/10Tests	3 pcs/10 pcs	Room temperature, 3 years
100 μm Cell Filter	3Tests/10Tests	3 pcs/10 pcs	Room temperature, 3 years

Documents

Manual

MSDS