Mycoplasma rapid detection kit（PCR）

Product Introduction

Background

The Mycoplasma detection kit has been carefully optimized by the Pricella's R&D team and has undergone long-term testing. It can be used to detect common Mycoplasma in biological products such as cell culture media, serum, and cell culture media, including Mycoplasma arginini, Mycoplasma hyorhinis, Mycoplasma orale, Mycoplasma pulmonis, Mycoplasma bovis and Mycoplasma yeatsii. This product is designed with specific primers for conserved sequences of common mycoplasma, which can directly use cell culture medium as a detection template to amplify mycoplasma DNA specifically. This product is easy to operate, has strong specificity, and high sensitivity.

Matters Need Attention

1. Ensure that all reagents are thoroughly thawed before use (refrigerate at 4℃ for 2 hours or leave at roomtemperature for 0.5 hours). After thawing, please separate (recommended 1000 rpm, 30 s) all components andmix well before use. For components with small amounts of liquid, centrifuge to collect the liquid fromthewall of the tube.
2. After the PCR system configuration is completed, it should be placed in the PCR machine for reaction as soonas possible, or temporarily stored in a refrigerator at 4℃ (within 6 hours). The product is ready for use andit isnot recommended to leave the pre-configured reaction system overnight.
3. Reagents should avoid repeated freezing and thawing as much as possible. If the product is stored at 4℃, please use it up within 3 months. If not in use for a long time, please freeze it at -5~-20℃.
4. The sample to be tested should be tested as soon as possible within 48 hours after sampling. If it cannot betested immediately, please freeze (-5~-20℃) and complete the test within one month.
5. Please wear a mask during operation to avoid contamination of the sample with mycoplasma carried in themouth, which may result in false positive results.
6. For samples with weak bands or other suspicious situations, the samples can be concentrated 10 times andretested for confirmation. Concentration operation: Centrifuge at 12000 rpm for 3 minutes to remove the supernatant

Add mycoplasma-free water (self prepared) of 1/10 volume of the original solution and mixwell again.
7. All products should be used within the shelf life. If they exceed the shelf life, they must be discarded.