Puromycin（10 mg/mL）

Product Introduction

Background

Puromycin is a kind of aminoglycoside antibiotic produced by the fermentative metabolism of Streptomyces albicans.
The mechanism of action of puromycin is to inhibit peptidyl transfer on prokaryotic and eukaryotic ribosomes; the pac gene found in Streptomyces alboniger encodes puromycin N-acetyltransferase (PAC), which confers resistance to puromycin. This feature is now commonly used in the selection of specific mammalian stably transfected cell lines carrying pac gene plasmids.

Ingredient Statement

Matters Need Attention

1. Puromycin is a toxic compound, please do a good job of relevant protection during operation.
2. This product has been filtered and sterilized by 0.1 μm filter, can be used directly after melting.
3. When using this product, attention should be paid to aseptic operation to avoid pollution.
4. The product should be thawed in 2-8℃, shake well after use, avoiding repeated freezing and thawing.
5. If there are precipitates after thawing, they can be vortexed and mixed evenly or blown with a pipette. After standing at room temperature for about 1 hour or at 37℃ in an incubator for 20-30 minutes, observe whether the precipitates can be dissolved normally, and if they can be dissolved, it can be used normally.
6. This product is a concentrated liquid, please dilute it as needed.
7. It is recommended to use the regular at 2-8℃ for preservation within one month. 6.It needs to be frozen at -5~-20℃ when not in use for a long time, and it is not suitable for long-term storage at room temperature or 2-8℃. To avoid repeated freezing and thawing, it is recommended to store it in small quantities after subpackaging.
8. This product is only for scientific research or further research use, not for diagnosis and treatment.