Rat Hepatic Stellate Cell Isolation and Culture Kit
P-CA-612
$ 518.00
Select a size
Quantity
In stock
Add to CartFor order requirements and technical needs, please Contact Us
Product Introduction
Background
The Rat Hepatic Stellate Cells Isolation and Culture Kit is specially developed for extracting primary Rat Hepatic Stellate Cells. As validated, standard operation using this kit enables the acquisition of one flask of cells (T-25 culture flask) per 1 Test,with a cell count exceeding 1 × 10⁶ cells. The cells can be passaged at a ratio of 1:2 for about 3 times. Through immunofluorescence identification, the cell purity (α - SMA positive rate) has been confirmed to exceed 90%.
Specifications
| Volume | 3Tests/10Tests |
| Valid Period | 12 months |
| Shipping Condition | Ice bag |
Matters Need Attention
1. Prior to formal experiments, it is recommended to conduct anatomical simulation training using 1-2 normal rats to familiarize operators with procedural workflows and improve tissue dissociation efficiency.
2. During the entire separation process, it is recommended to place the culture dish containing the tissue on an ice plate (2-8℃) to maintain low temperature, but be careful not to freeze the tissue and liquid due to low temperature, so as not to damage the tissue and affect the extraction efficiency.
3. Reagent preparation or dispensing must strictly adhere to aseptic technique protocols. After dispensing, seal the containers immediately with a sealing film, use them promptly to avoid repeated freeze-thaw cycles orcontamination.
Kit Components
| Name | Size | Appearance | Storage and Expiration Dat |
| Specialized Washing Solution For Rat Hepatic Stellate Cells | 3Tests/10Tests | 250 mL/500 mL | 2-8℃, 1 year |
| Diluent of Digestive Enzyme For Rat Hepatic Stellate Cells | 3Tests/10Tests | 15 mL/50 mL | 2-8℃, 1 year |
| Specific Digestive Enzyme For Rat Hepatic Stellate Cells | 3Tests/10Tests | 1.5 mL/5 mL | -5~-20℃, 1 year |
| Specific Separation Solution A For Rat Hepatic Stellate Cells | 3Tests/10Tests | 15 mL/50 mL | 2-8℃,shading light,1 year |
| Specific Separation Solution B For Rat Hepatic Stellate Cells | 3Tests/10Tests | 15 mL/50 mL | 2-8℃,shading light,1 year |
| Basic Culture Medium For Rat Hepatic Stellate Cells | 3Tests/10Tests | 150 mL/500 mL | 2-8℃, 1 year |
| Supplement For Rat Hepatic Stellate Cells | 3Tests/10Tests | 15 mL/50 mL | -5~-20℃, 1 year |
| 100 μm Cell Filter | 3Tests/10Tests | 3 pcs/10 pcs | Room temperature, 3 years |
| 70 μm Cell Filter | 3Tests/10Tests | 3 pcs/10 pcs | Room temperature, 3 years |
