Rat Intervertebral Disc Annulus Fibrosus Cell Isolation and Culture Kit

Cat. No.: P-CA-626

P-CA-626

$ 680.00

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3Tests 10Tests

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Product Introduction Documents

Product Introduction

Background

The Rat Intervertebral Disc Fibroblast Isolation and Culture Kit is specifically developed for the extraction of primary Rat Intervertebral Disc Fibroblasts. Verified through standardized procedures, each 1 Test of this kit enables the acquisition of one flask of cells (T-25 culture flask), with a cell count exceeding 1×10⁶ cells. When subcultured at a 1:2 ratio, the cells can undergo 3-5 passages, with the best cell state within the first 3 passages. Through immunofluorescence analysis, the cell purity (Collagen I positive rate) has been confirmed to exceed 90%.

Specifications

Volume	3Tests/10Tests
Valid Period	12 months
Shipping Condition	Ice bag

Matters Need Attention

1. Prior to formal experiments, it is recommended to conduct anatomical simulation training using 1-2 normal rats to familiarize yourself with operational procedures and improve tissue isolation efficiency.
2. Reagent preparation or aliquoting must strictly adhere to aseptic techniques. After dispensing, seal the containers immediately with a sealing film, use them promptly to avoid repeated freeze-thaw cycles or contamination.

Kit Components

Name	Size	Appearance	Storage and Expiration Dat
Specialized Washing Solution For Rat Intervertebral Disc Fibroblast Cells	3Tests/10Tests	250 mL/500 mL×2	2-8℃, 1 year
Specialized Digestive Solution For Rat Intervertebral Disc Fibroblast Cells	3Tests/10Tests	15 mL/50 mL	-5~-20℃, 1 year
Basic Culture Medium For Rat Intervertebral Disc Fibroblast Cells	3Tests/10Tests	50 mL/100 mL	2-8℃, 1 year
Supplement For Rat Intervertebral Disc Fibroblast Cells	3Tests/10Tests	10 mL/20 mL	-5~-20℃, 1 year
100 μm Cell Filter	3Tests/10Tests	3 pcs/10 pcs	Room temperature, 3 years
70 μm Cell Filter	3Tests/10Tests	3 pcs/10 pcs	Room temperature, 3 years

Documents

Manual

MSDS