PB180423
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Product Introduction
Background
Trypan Blue is a cell-reactive dye that is often used to test the integrity of cell membranes and cell survival. Trypan blue staining is one of the most commonly used methods for dead cell identification in tissue and cell culture.Cells that are inactive or have incomplete cell membranes can be stained blue by trypan blue while normal living cells have intact cell membranes that reject trypan blue and do not dye cells blue. The loss of cell membrane integrity is generally considered to be that the cell has died. Macrophages can phagocytosis trypan blue, so it can be used as a living staining agent for macrophages.In addition, it should be noted that apoptotic bodies also have trypan blue staining rejection.
Ingredient Statement
With
• 0.85% NaCl Dissolvent
• 4 g/L Trypan Blue Solution
Matters Need Attention
1. This product is for research use only.
2. The time of staining should be appropriate, otherwise the living cells will gradually accumulate the dye and become dyed, which will cause the deviation of the test results.
Specifications
| Concentration | 4 g/L |
| Volume | 100mL |
| Form | Liquid |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 36 months |
| Storage Condition | This product can be stored at 2-30℃ for 36 months. |
| Shipping Condition | Room Temperature |
Publications
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025) IF: 7.7
DOI: 10.1016/j.ijbiomac.2025.140071
Product Cited: 0.4% Trypan Blue Solution
Journal: CELLULAR AND MOLECULAR LIFE SCIENCES (2025) IF: 6.2
DOI: 10.1007/s00018-025-05673-1
Product Cited: Mouse Embryonic Lung Fibroblast Cells, 0.4% Trypan Blue Solution, Mouse Embryonic Lung Fibroblast Cell Complete Medium
Journal: JOURNAL OF MOLECULAR NEUROSCIENCE (2025) IF: 2.8
DOI: 10.1007/s12031-025-02356-1
Product Cited: TUNEL In Situ Apoptosis Kit (HRP-DAB Method), 0.4% Trypan Blue Solution, Annexin V-FITC/PI Apoptosis Kit
Journal: JOURNAL OF COLLOID AND INTERFACE SCIENCE (2024) IF: 9.4
DOI: 10.1016/j.jcis.2024.12.097
Product Cited: 0.4% Trypan Blue Solution
Journal: AQUACULTURE (2024) IF: 4.5
DOI: 10.1016/j.aquaculture.2024.740849
Product Cited: 0.4% Trypan Blue Solution
FAQs
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Q:1What causes trypan blue to form a precipitate?
AnswerExposure to light may degrade the dye and these contaminants may promote precipitation. Trypan blue can also form orange/red fibrous aggregates if exposed to refrigeration or freezing temperatures.
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Q:2Prior to staining with trypan blue solution, do the cells need to be washed to remove the culture medium?
AnswerTrypan blue will bind to serum proteins as well as cellular proteins, which may result a high level of background staining. If the background is too dark, cells should be pelleted and resuspended in protein-free medium, buffer, or normal saline prior to counting.
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Q:3How does trypan blue solution stain viable cells?
AnswerTrypan blue is a cell impermeant stain used to estimate the number of dead cells in a viable population. Its utility is based on the fact that it is a charged dye and does not enter cells unless the membrane is compromised. Live (viable) cells exclude the dye but dead (non-viable) cells or cells with a compromised membrane are stained an intense blue.
