1 M HEPES Solution

Cat. No.: PB180325 Publications(62) Manual

PB180325

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100mL

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Product Introduction Documents Publications

Product Introduction

Background

HEPES is all known as N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, is a commonly used biological buffer. pKa (25℃) is 7.5 and an effective buffer range of pH is 6.8-8.2. The greatest advantage of HEPES is that to maintain the pH range of a solution for a longer period of time in an open environment.
HEPES cannot pass through biofilms. It is a chemical and enzymatic stable substance with limited effect on biochemical reactions, and the absorption of visible and ultraviolet light is extremely low. Therefore, it is generally considered that HEPES is an excellent biological buffer, and there is no toxic effect to the cells. The working concentration is 10-50 mM and the final concentration for cell culture is 10 mM.

Ingredient Statement

With

• 238.3 g/L HEPES

• Purified Water Dissolvent

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the pollution.
4. This product is 50 × concentrated solution and dilute it to to the desired concentration before use.

Specifications

Concentration	1 M
Volume	100mL
Form	Liquid
Bacteria Detection	Negative
Fungi Detection	Negative
Mycoplasma Detection	Negative
Endotoxin Content	< 3 EU/mL
pH	7.2-7.4
Animal Origin Ingredient	Without
Green Features	Sustainable packaging
Valid Period	24 months
Storage Condition	This product can be stored at 2-8℃ for 24 months
Shipping Condition	Ice bag