PM150312
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Product Introduction
Background
The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3151 mg/L D-Glucose
• 2.5 mM L-Glutamine
• 2438 mg/L NaHCO₃
• 8.1 mg/L Phenol red
• 0.5 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Publications
Journal: FOOD RESEARCH INTERNATIONAL (2024) IF: 7.0
DOI: 10.1016/j.foodres.2024.115073
Product Cited: DMEM/F12 Medium
Journal: Cell Death Discovery (2024) IF: 7.0
DOI: 10.1038/s41420-024-01813-x
Product Cited: DMEM/F12
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024) IF: 6.8
DOI: 10.1016/j.ecoenv.2024.115989
Product Cited: KGN Cells Line, DMEM/F12, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×
Journal: JOURNAL OF MEDICINAL CHEMISTRY (2024) IF: 6.8
DOI: 10.1021/acs.jmedchem.4c02034
Product Cited: MEM Medium, RPMI-1640 Medium, DMEM (High glucose) Medium, A2780 Cells Line, Hep G2 Cells Line, DMEM/F12 Medium, MCF7 [MCF-7] Cells Line, HK-2 Cells Line, Human Umbilical Vein Endothelial Cells, HCT 116 Cells Line
Journal: EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2024) IF: 6.7
DOI: 10.1016/j.ejmech.2024.116395
Product Cited: DMEM (High glucose) Medium, RPMI-1640 Medium, Penicillin-Streptomycin Solution, 100 ×, Fetal Bovine Serum, DMEM/F12 Medium
