PM150110
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Product Introduction
Background
RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media.
RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.
Ingredient Statement
With
• 2000 mg/L D-Glucose
• 2 mM L-Glutamine
• 2000 mg/L NaHCO₃
• 5 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
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Publications
Journal: EMBO JOURNAL (2025) IF: 9.4
DOI: 10.1038/s44318-024-00359-z
Product Cited: MCF 10A Cells Line, MDA-MB-468-06 Cells Line, MCF 10A Cell Complete Medium, MDA-MB-453 Cells Line, 293T [HEK-293T] Cells Line, MCF7 [MCF-7] Cell Complete Medium, MDA-MB-231 Cells Line, RPMI-1640 Medium, MCF7 [MCF-7] Cells Line
Journal: PHARMACOLOGICAL RESEARCH (2025) IF: 9.1
DOI: 10.1016/j.phrs.2025.107643
Product Cited: RPMI-1640, without L-glutamine Medium, DMEM (High glucose) Medium, RPMI-1640 Medium
Journal: Materials Today Bio (2025) IF: 8.7
DOI: 10.1016/j.mtbio.2025.101867
Product Cited: Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100 ×, RPMI-1640 Medium, 0.05% Trypsin Solution (dissolved in PBS), Phosphate Buffer (PBS, 1 ×)
Journal: ACTA PHARMACOLOGICA SINICA (2025) IF: 8.4
DOI: 10.1038/s41401-025-01599-3
Product Cited: 200 mM L-Glutamine Solution, 100 ×, DMEM (High glucose) Medium, RPMI-1640 Medium, Penicillin-Streptomycin Solution, 100 ×
Journal: International Journal of Biological Sciences (2025) IF: 8.2
DOI: 10.7150/ijbs.105265
Product Cited: DMEM (High glucose) Medium, RPMI-1640 Medium, Fetal Bovine Serum
