DMEM/F12

Cat. No.: PM150312 Publications(317) Manual

PM150312

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500mL

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Product Introduction Related Products Documents Publications

Product Introduction

Background

The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Ingredient Statement

With

• 3151 mg/L D-Glucose

• 2.5 mM L-Glutamine

• 2438 mg/L NaHCO₃

• 8.1 mg/L Phenol red

• 0.5 mM Sodium pyruvate

Without

• HEPES

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

System Certification

GMP Production and Quality System
This product is manufactured in a GMP-compliant factory, formulated with water for injection, and has obtained ISO 9001 and ISO 13485 certifications.

Specifications

Concentration	1×
Volume	500mL
Form	Liquid
Bacteria Detection	Negative
Fungi Detection	Negative
Mycoplasma Detection	Negative
Endotoxin Content	< 3 EU/mL
pH	7.2-7.4
Medium	Components 3151 mg/L D-Glucose 2.5 mM L-Glutamine 2438 mg/L NaHCO₃ 0.5 mM Sodium Pyruvate 8.1 mg/L Phenol Red
Animal Origin Ingredient	Without
Green Features	Sustainable packaging
Valid Period	24 months
Storage Condition	This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition	Room Temperature