PB180429
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Product Introduction
Background
Insulin (INS) is a double-stranded (α, β) polypeptide hormone secreted by islet beta cells. It is the only known hormone in the body that reduces blood sugar and simultaneously promotes glycogen, fat, and protein synthesis.
Transferrin is an indispensable component in body fluids. It is not only involved in iron transport and metabolism, respiration, cell proliferation and immune system regulation, but also can regulate iron ion balance and energy balance. And it has antibacterial and bactericidal function.
Sodium Selenite is a cofactor that provides glutathione peroxidase and other proteins and is used as an antioxidant in culture.
Originally used in combination with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G solutions are now commonly used in combination with other basal medium to support multiple cell types in the presence of 2-4% FBS.
ITS is formulated with Earle balanced salt solution (EBSS). Pricella offers three different ITS: ITS-G for general use; ITS-A for adherent cell culture; and ITS-X for cell types requiring aminoethanol, such as bronchial epithelial cells.
Ingredient Statement
With
• EBSS Dissolvent
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
4. This product is 100 × concentrated solution. Please dilute it to to the desired concentration before use.
Specifications
| Concentration | 100× |
| Volume | 10mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.8-8.3 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 18 months |
| Storage Condition | This product can be stored at 2-8℃ for 18 months. |
| Shipping Condition | Ice bag |
Publications
Journal: FREE RADICAL BIOLOGY AND MEDICINE (2025) IF: 8.2
DOI: 10.1016/j.freeradbiomed.2025.06.039
Product Cited: Insulin-Transferrin-Selenium (ITS-G), 100 ×, Fetal Bovine Serum, AML12 Cells Line
Journal: Cell Death & Disease (2025) IF: 8.1
DOI: 10.1038/s41419-025-07376-8
Product Cited: Insulin-Transferrin-Selenium (ITS-G), 100 ×
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2025) IF: 4.9
DOI: 10.3390/ijms26114978
Product Cited: Insulin-Transferrin-Selenium (ITS-G), 100 ×
Journal: HYPERTENSION RESEARCH (2025) IF: 4.3
DOI: 10.1038/s41440-025-02143-w
Product Cited: Insulin-Transferrin-Selenium (ITS-G), 100 ×, 10 mg/mL Gentamicin Sulphate Solution, DMEM/F12 Medium
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2025) IF: 4.2
DOI: 10.1016/j.bbadis.2025.167815
Product Cited: Insulin-Transferrin-Selenium (ITS-G), 100 ×, DMEM/F12 Medium, Fetal Bovine Serum
FAQs
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Q:1Is Insulin-Transferrin-Selenium (ITS -G) (100X) animal origin free?
AnswerInsulin-Transferrin-Selenium (ITS -G) is animal origin free.
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Q:2What is the Insulin source in the Insulin-Transferrin-Selenium (ITS) supplements?
AnswerRecombinant Human Insulin is used in the ITS supplements.
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Q:3What is the advantage of using Insulin-Transferrin-Selenium (ITS) supplements in cultures?
AnswerITS supplements are used to reduce the amount of fetal bovine serum (FBS) required for routine maintenance and low-density attachment of many cell types.
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Q:4What is the advantage of using Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) Supplement in cultures?
AnswerITS-A Supplement is designed as a supplement for RPMI- 1640 and Earle's Minimal Essential Medium (EMEM), and will enhance the growth of various adherent cell types at FBS concentrations < 4%.
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Q:5Can I store aliquots of Insulin-Transferrin-Selenium (ITS -G) (100X)at -20 degrees C without compromising integrity of the product?
AnswerWe recommend storing the product at 2-8℃ as there is a chance that the insulin will precipitate upon freeze-thaw.
