PM150312
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Product Introduction
Background
The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3151 mg/L D-Glucose
• 2.5 mM L-Glutamine
• 2438 mg/L NaHCO₃
• 8.1 mg/L Phenol red
• 0.5 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
This product is manufactured in a GMP-compliant factory, formulated with water for injection, and has obtained ISO 9001 and ISO 13485 certifications.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Medium | Components 3151 mg/L D-Glucose 2.5 mM L-Glutamine 2438 mg/L NaHCO₃ 0.5 mM Sodium Pyruvate 8.1 mg/L Phenol Red |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
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Publications
Journal: EXPERT OPINION ON THERAPEUTIC TARGETS (2023) IF: 5.8
DOI: 10.1080/14728222.2023.2263908
Product Cited: U-2 OS [U2OS] Cells Line, Saos-2 Cells Line, MG-63 Cells Line, HOS Cells Line, SJSA-1 Cells Line, hFOB 1.19 Cells Line, McCoy's 5A, MEM, RPMI-1640, DMEM/F12
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.600
DOI: 10.1016/j.intimp.2022.109605
Product Cited: AML12 Cells Line, Penicillin-Streptomycin Solution, 100×, DMEM/F12, Mouse Hepatic Stellate cells, Mouse Hepatic Stellate cells Complete Medium
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 5.6
Product Cited: KGN Cells Line, DMEM/F12
Journal: Biology Direct (2023) IF: 5.500
Journal: AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY (2023) IF: 5.5
DOI: 10.1152/ajpcell.00249.2023
Product Cited: MDA-MB-231 Cells Line, MDA-MB-468 Cells Line, MDA-MB-157 Cells Line, BT-549 Cells Line, 293 [HEK-293] Cells Line, DMEM/F12, Hs 578T Cells Complete Medium
