PM150312
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Product Introduction
Background
The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3151 mg/L D-Glucose
• 2.5 mM L-Glutamine
• 2438 mg/L NaHCO₃
• 8.1 mg/L Phenol red
• 0.5 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Publications
Journal: Small Methods (2023) IF: 12.4
Product Cited: DMEM/F12, Donor Equine Serum, EGF/Urogastrone/URG, Human, Recombinant, 10 mg/mL Recombinant Human Insulin Solution, MEM Non-Essential Amino Acid Solution (NEAA), 100×, Hela Cells Line, MCF 10A Cells Line
Journal: Advanced Healthcare Materials (2023) IF: 10.000
Product Cited: Rat Neural Stem cells, Rabbit Alveolar Macrophage cells, RPMI-1640, DMEM/F12
Journal: Molecular & Cellular Toxicology (2023) IF: 1.7
DOI: 10.1007/s13273-023-00368-9
Product Cited: MCF 10A Cells Line, DMEM/F12, Donor Equine Serum, Penicillin-Streptomycin Solution, 100×, MDA-MB-231 Cells Line, MDA-MB-468 Cells Line, DMEM(High glucose), BT-549 Cells Line, RPMI-1640, MDA-MB-436 Cells Line
Journal: NEUROCHIRURGIE (2023) IF: 1.600
DOI: 10.1016/j.neuchi.2023.101426
Product Cited: BV2 Cells Line, DMEM/F12
Journal: JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH (2023) IF: 1.6
DOI: 10.1111/jog.15752
Product Cited: DMEM/F12 Medium
