PM150312
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Product Introduction
Background
The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3151 mg/L D-Glucose
• 2.5 mM L-Glutamine
• 2438 mg/L NaHCO₃
• 8.1 mg/L Phenol red
• 0.5 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Publications
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY (2025) IF: 4.8
DOI: 10.1016/j.intimp.2025.114015
Product Cited: DMEM/F12 Medium, RPMI-1640 Medium, DMEM (High glucose) Medium
Journal: Drug Design Development and Therapy (2025) IF: 4.7
DOI: 10.2147/DDDT.S514209
Product Cited: Fetal Bovine Serum, DMEM/F12 Medium, Rat Corpora Cavernous Smooth Muscle Cells
Journal: iScience (2025) IF: 4.6
DOI: 10.1016/j.isci.2025.112526
Product Cited: Donor Equine Serum, BT-549 Cells Line, DMEM/F12 Medium, MCF 10A Cells Line, MDA-MB-231 Cells Line, Penicillin-Streptomycin Solution, 100 ×, RPMI-1640 Medium, 10 mg/mL Recombinant Human Insulin Solution, MEM Non-Essential Amino Acid Solution (NEAA), 100 ×, HCC1937 Cells Line, MDA-MB-468-06 Cells Line, Leibovitz's L-15 Medium, EGF/Urogastrone/URG, Human, Recombinant, Fetal Bovine Serum
Journal: EXPERIMENTAL NEUROLOGY (2025) IF: 4.6
DOI: 10.1016/j.expneurol.2025.115235
Product Cited: DMEM/F12 Medium
Journal: Frontiers in Pharmacology (2025) IF: 4.4
DOI: 10.3389/fphar.2025.1543575
Product Cited: Penicillin-Streptomycin Solution, 100 ×, DMEM/F12 Medium, Fetal Bovine Serum
