PM150270
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Product Introduction
Background
DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times.
The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucoser type), which has been widely used in cell culture.
DMEM medium without glucose can be added to the concentration of glucose at will according to the research needs, which is convenient and quick.
DMEM (glucose free) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 4 mM L-Glutamine
• 3700 mg/L NaHCO₃
• 15 mg/L Phenol red
• 1 mM Sodium pyruvate
Without
• D-Glucose
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: Materials Today Bio (2025) IF: 8.7
DOI: 10.1016/j.mtbio.2025.101704
Product Cited: HT22 Cells Line, DMEM (Glucose free) Medium, BV2 Cells Line
Journal: FREE RADICAL BIOLOGY AND MEDICINE (2025) IF: 7.1
DOI: 10.1016/j.freeradbiomed.2025.01.016
Product Cited: DMEM (Glucose free) Medium
Journal: ACTA PHARMACOLOGICA SINICA (2025) IF: 6.9
DOI: 10.1038/s41401-025-01555-1
Product Cited: DMEM (Glucose free) Medium
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2025) IF: 6.2
DOI: 10.1016/j.ecoenv.2025.118113
Product Cited: DMEM (Glucose free) Medium, LX-2 Cells Line, Fetal Bovine Serum
Journal: CELLULAR AND MOLECULAR LIFE SCIENCES (2025) IF: 6.2
DOI: 10.1007/s00018-025-05689-7
Product Cited: Fetal Bovine Serum, bEnd.3 [BEND3] Cells Line, Hank's Balanced Salt Solution (HBSS), with calcium, magnesium, without phenol red, DMEM (Glucose free) Medium