PM150270
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Product Introduction
Background
DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times.
The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucoser type), which has been widely used in cell culture.
DMEM medium without glucose can be added to the concentration of glucose at will according to the research needs, which is convenient and quick.
DMEM (glucose free) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 4 mM L-Glutamine
• 3700 mg/L NaHCO₃
• 15 mg/L Phenol red
• 1 mM Sodium pyruvate
Without
• D-Glucose
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: MOLECULAR NEUROBIOLOGY (2025) IF: 4.6
DOI: 10.1007/s12035-025-04952-z
Product Cited: bEnd.3 [BEND3] Cells Line, DMEM (Glucose free) Medium
Journal: CELLULAR SIGNALLING (2025) IF: 4.4
DOI: 10.1016/j.cellsig.2025.111719
Product Cited: DMEM (Glucose free) Medium
Journal: Frontiers in Pharmacology (2025) IF: 4.4
DOI: 10.3389/fphar.2025.1583509
Product Cited: DMEM (Glucose free) Medium, H9c2(2-1) Cells Line, Penicillin-Streptomycin Solution, 100 ×, Fetal Bovine Serum, DMEM (High glucose) Medium
Journal: Frontiers in Pharmacology (2025) IF: 4.4
DOI: 10.3389/fphar.2025.1525605
Product Cited: DMEM (High glucose) Medium, DMEM (Glucose free) Medium
Journal: Frontiers in Pharmacology (2025) IF: 4.4
DOI: 10.3389/fphar.2024.1532610
Product Cited: MEM, with NEAA Medium, DMEM (Glucose free) Medium, U-2 OS [U2OS] Cells Line, McCoy's 5A Medium, HOS Cells Line