Ham's F-12 Complete Medium (10)

Cat. No.: PM150810B Publications(2) Manual
Ham's F-12 Complete Medium (10) - 1
  • Ham's F-12 Complete Medium (10)Ham's F-12 Complete Medium (10) - 1

PM150810B

$ 108.00

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125mL×4

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Product Introduction

Background

Ham's F-12 Nutrient Mixture was designed by Ham in 1969 based on Ham's F-10 nutrient mixture, which was originally used for serum-free culture of CHO cells. Ham's F-12 is often used as the basic culture medium for serum free culture. At low serum levels, it is especially suitable for single cell culture and clonal culture. After adding serum, it is also widely used in the culture of cancer cells and primary cells, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts, and chicken embryonic cells. In addition, the DMEM/F12 medium obtained  by mixing Ham's F-12 in equal volume with DMEM is more nutritious and widely used.

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture.
4. It is not suitable for long time storage at room temperature.
5. This product is a ready-to-use medium. If there is no special need, don't add serum, penicillin and streptomycin. It can be used directly.

Specifications
Concentration
Volume 125mL×4
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.4-7.8
Green Features Sustainable packaging
Valid Period 6 months
Storage Condition This product can be stored at 2-8℃ for 6 months with shading light.
Shipping Condition Ice bag

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Documents

Publications

Circ_0006220 (circ-TADA2A) accelerates prostate cancer cell malignant behaviors through miR-520f-3p/CDCA7 axis

Journal: CELLULAR AND MOLECULAR BIOLOGY (2024) IF: 1.5

DOI: 10.14715/cmb/2024.70.7.12

Product Cited: Ham's F-12 Complete Medium (10), PC-3 Cells Line, VCaP Cells Line, DU 145 Cells Line, SILAC RPMI-1640 Medium, DMEM (High glucose) Complete Medium (10), RWPE-1 Cells Line, 22RV1 Cells Line

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