PM150810
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Product Introduction
Background
Ham's F-12 Nutrient Mixture was designed by Ham in 1969 based on Ham's F-10 nutrient mixture, which was originally used for serum-free culture of CHO cells. Ham's F-12 is often used as the basic culture medium for serum free culture. At low serum levels, it is especially suitable for single cell culture and clonal culture. After adding serum, it is also widely used in the culture of cancer cells and primary cells, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts, and chicken embryonic cells. In addition, the DMEM/F12 medium obtained by mixing Ham's F-12 in equal volume with DMEM is more nutritious and widely used.
Ingredient Statement
With
• 1802 mg/L D-Glucose
• 1 mM L-Glutamine
• 1176 mg/L NaHCO₃
• 1.2 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.0-7.6 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
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Publications
Journal: ENVIRONMENT INTERNATIONAL (2023) IF: 11.8
DOI: 10.1016/j.envint.2023.108407
Product Cited: SV-HUC-1 Cells Line, Ham's F-12
Journal: Current Research in Food Science (2022) IF: 6.300
DOI: 10.1016/j.crfs.2022.10.018
Product Cited: Ham's F-12, Human Umbilical Vein Endothelial cells
Journal: Journal of Oncology (2022) IF: 4.500
DOI: 10.1155/2022/9124142
Product Cited: KYSE-30 Cells Line, KYSE-150 Cells Line, RPMI-1640, Ham's F-12, Penicillin-Streptomycin Solution, 100×, Annexin V-FITC/PI Apoptosis Kit
Journal: JOURNAL OF CLINICAL LABORATORY ANALYSIS (2022) IF: 3.100
DOI: 10.1002/jcla.24633
Product Cited: Ham's F-12, Fetal Bovine Serum
Journal: INTERNATIONAL JOURNAL OF NEUROSCIENCE (2022) IF: 2.590
DOI: 10.1080/00207454.2022.2126772
Product Cited: Ham's F-12 Medium, MEM Non-Essential Amino Acid Solution (NEAA), 100 ×
