Ham's F-12

Cat. No.: PM150810 Publications(54) Manual
Ham's F-12 - 1
  • Ham's F-12Ham's F-12 - 1

PM150810

$ 10.00

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500mL

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Product Introduction

Background

Ham's F-12 Nutrient Mixture was designed by Ham in 1969 based on Ham's F-10 nutrient mixture, which was originally used for serum-free culture of CHO cells. Ham's F-12 is often used as the basic culture medium for serum free culture. At low serum levels, it is especially suitable for single cell culture and clonal culture. After adding serum, it is also widely used in the culture of cancer cells and primary cells, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts, and chicken embryonic cells. In addition, the DMEM/F12 medium obtained  by mixing Ham's F-12 in equal volume with DMEM is more nutritious and widely used.

Ingredient Statement
With

• 1802 mg/L D-Glucose

• 1 mM L-Glutamine

• 1176 mg/L NaHCO₃

• 1.2 mg/L Phenol red

Without

• HEPES

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

Specifications
Concentration
Volume 500mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.0-7.6
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Documents

Publications

PMEPA1 promotes gastric cancer cell proliferation by regulating the ubiquitin-mediated degradation of 14-3-3σ and promoting cell cycle progression

Journal: BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH (2024) IF: 1.9

DOI: 10.1590/1414-431X2024e13985

Product Cited: 293T [HEK-293T] Cells Line, MKN-7 [MKN7; MKN 7] Cells Line, DMEM (High glucose) Medium, AGS Cells Line, BGC-823 Cells Line, HGC-27 Cells Line, MKN-45 Cells Line, Ham's F-12 Medium, RPMI-1640 Medium, GES-1 Cells Line

PTPMT1 inhibition induces apoptosis and growth arrest of human SCLC cells by disrupting mitochondrial metabolism

Journal: Translational Cancer Research (2024) IF: 1.5

DOI: 10.21037/tcr-2024-2379

Product Cited: Ham's F-12 Medium, NCI-H69 Cells Line, A549 [A-549] Cells Line

KIRREL promotes the proliferation of gastric cancer cells and angiogenesis through the PI3K/AKT/mTOR pathway

Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2023) IF: 5.3

DOI: 10.1111/jcmm.18020

Product Cited: Ham's F-12

Design, synthesis, antibacterial/antitumor activity and in vitro stability of novel cordycepin derivatives with unsaturated fatty acid chain

Journal: EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES (2023) IF: 4.600

DOI: 10.1016/j.ejps.2023.106466

Product Cited: Hela Cells Line, A549 [A-549] Cells Line, MCF7 [MCF-7] Cells Line, SMMC-7721 Cells Line, DMEM(High glucose), Penicillin-Streptomycin Solution, 100×, Ham's F-12

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