PM150910
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Product Introduction
Background
The Ham's F-12K medium increases the concentration of amino acids and pyruvic acid on the basis of Ham's F-12, reduces the concentration of glucose, and modifies the composition and content of the salt.The Ham's F-12K medium was initially designed to culture primary human hepatocytes and differentiated cells of rats and chickens.
The Ham's F-12K medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1260 mg/L D-Glucose
• 2 mM L-Glutamine
• 2500 mg/L NaHCO₃
• 3 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY (2024) IF: 4.8
DOI: 10.1016/j.jbc.2024.107152
Product Cited: PC-3 Cells Line, DU 145 Cells Line, 22RV1 Cells Line, Ham's F-12K, MEM(with NEAA), RPMI-1640
Journal: Pharmaceuticals (2024) IF: 4.6
DOI: 10.3390/ph17050582
Product Cited: Ham's F-12K Medium, Phosphate Buffer (PBS, 1 ×)
Journal: Scientific Reports (2024) IF: 4.6
DOI: 10.1038/s41598-024-60000-1
Product Cited: NCI-H1437 Cells Line, A549 [A-549] Cells Line, Fetal Bovine Serum, Ham's F-12K Medium, NCI-H1299 Cells Line
Journal: MOLECULES (2024) IF: 4.6
DOI: 10.3390/molecules29040837
Product Cited: A549 [A-549] Cells Line, NCI-H1437 Cells Line, NCI-H1299 Cells Line, Ham's F-12K, Fetal Bovine Serum
Journal: ENVIRONMENTAL TOXICOLOGY (2024) IF: 4.5
DOI: 10.1002/tox.24278
Product Cited: DMEM/F12 Medium, Ham's F-12K Medium, McCoy's 5A Medium