PM150910
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Product Introduction
Background
The Ham's F-12K medium increases the concentration of amino acids and pyruvic acid on the basis of Ham's F-12, reduces the concentration of glucose, and modifies the composition and content of the salt.The Ham's F-12K medium was initially designed to culture primary human hepatocytes and differentiated cells of rats and chickens.
The Ham's F-12K medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1260 mg/L D-Glucose
• 2 mM L-Glutamine
• 2500 mg/L NaHCO₃
• 3 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: MOLECULAR BIOTECHNOLOGY (2024) IF: 2.4
DOI: 10.1007/s12033-024-01323-2
Product Cited: Ham's F-12K Medium, PC-9 Cells Line, A549 [A-549] Cells Line, RPMI-1640 Medium, Fetal Bovine Serum
Journal: BMC GASTROENTEROLOGY (2024) IF: 2.4
DOI: 10.1186/s12876-024-03248-1
Product Cited: Fetal Bovine Serum, Ham's F-12K Medium, AR42J Cells Line
Journal: Thoracic Cancer (2024) IF: 2.3
Product Cited: Ham's F-12K Medium
Journal: MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS (2024) IF: 2.3
DOI: 10.1016/j.mrfmmm.2024.111876
Product Cited: Fetal Bovine Serum, 5637 Cells Line, RPMI-1640 Medium, McCoy's 5A Medium, T24 [T-24] Cells Line, Ham's F-12K Medium, J82 Cells Line, DMEM (High glucose) Medium, MEM, with NEAA Medium, 293T [HEK-293T] Cells Line, Penicillin-Streptomycin Solution, 100 ×, SV-HUC-1 Cells Line, RT4 Cells Line
Journal: Cell Division (2024) IF: 2.3
DOI: 10.1186/s13008-024-00112-2
Product Cited: A549 [A-549] Cells Complete Medium, Ham's F-12K, HCC827 Cells Complete Medium, RPMI-1640