PM150910
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Product Introduction
Background
The Ham's F-12K medium increases the concentration of amino acids and pyruvic acid on the basis of Ham's F-12, reduces the concentration of glucose, and modifies the composition and content of the salt.The Ham's F-12K medium was initially designed to culture primary human hepatocytes and differentiated cells of rats and chickens.
The Ham's F-12K medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1260 mg/L D-Glucose
• 2 mM L-Glutamine
• 2500 mg/L NaHCO₃
• 3 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: CURRENT PROTEIN & PEPTIDE SCIENCE (2024) IF: 1.9
DOI: 10.2174/0113892037273375231204080906
Product Cited: BEAS-2B Cells Line, Ham's F-12K Medium, DMEM (High glucose) Medium, RPMI-1640 Medium
Journal: BMC Urology (2024) IF: 1.7
DOI: 10.1186/s12894-024-01666-7
Product Cited: DU 145 Cells Line, Ham's F-12K Medium, PC-3 Cells Line, MEM, with NEAA Medium
Journal: CANCER LETTERS (2023) IF: 9.700
DOI: 10.1016/j.canlet.2023.216058
Product Cited: SV-HUC-1 Cells Line, UM-UC-3 Cells Line, J82 Cells Line, 5637 Cells Line, SW 780 [SW-780, SW780] Cells Line, T24 [T-24] Cells Line, TCCSUP Cells Line, Ham's F-12K, MEM(with NEAA), Leibovitz's L-15, McCoy's 5A
Journal: Cardiovascular Diabetology (2023) IF: 9.300
DOI: 10.1186/s12933-023-01818-3
Product Cited: Ham's F-12K
Journal: ACS Sensors (2023) IF: 8.900
DOI: 10.1021/acssensors.2c01564
Product Cited: A549 [A-549] Cells Line, Fetal Bovine Serum, Hela Cells Line, MDA-MB-231 Cells Line, Penicillin-Streptomycin Solution, 100 ×, DMEM (High glucose) Medium, Ham's F-12K Medium