PM150910
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Product Introduction
Background
The Ham's F-12K medium increases the concentration of amino acids and pyruvic acid on the basis of Ham's F-12, reduces the concentration of glucose, and modifies the composition and content of the salt.The Ham's F-12K medium was initially designed to culture primary human hepatocytes and differentiated cells of rats and chickens.
The Ham's F-12K medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1260 mg/L D-Glucose
• 2 mM L-Glutamine
• 2500 mg/L NaHCO₃
• 3 mg/L Phenol red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: Molecular and Cellular Biochemistry (2022) IF: 4.300
DOI: 10.1007/s11010-021-04320-0
Product Cited: 16HBE Cells Line, A549 [A-549] Cells Line, NCI-H1299 Cells Line, RPMI-1640, Ham's F-12K, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×, PUMC-HUVEC-T1 Cells Line
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY (2022) IF: 3.800
DOI: 10.1007/s11010-022-04646-3
Product Cited: A549 [A-549] Cells Line, NCI-H292 Cells Line, Ham's F-12K, RPMI-1640, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×
Journal: Annals of Translational Medicine (2022) IF: 3.616
DOI: 10.21037/atm-22-4652
Product Cited: Human Umbilical Vein Endothelial Cells, Endothelial growth factor (100 × ), Ham's F-12K Medium
Journal: Biomed Research International (2022) IF: 3.300
DOI: 10.1155/2022/7841279
Product Cited: A549 [A-549] Cells Line, NCI-H1299 Cells Line, Ham's F-12K, RPMI-1640
Journal: Applied Biological Chemistry (2022) IF: 3.200
DOI: 10.1186/s13765-022-00726-6
Product Cited: AGS Cells Line, HGC-27 Cells Line, GES-1 Cells Line, Ham's F-12K