PM151010
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Product Introduction
Background
The buffer system of Leibovitz's L-15 medium is composed of phosphate and free amino acids, instead of the traditional sodium bicarbonate buffer system. Meanwhile, the substitution of galactose and sodium pyruvate for glucose prevents prevents the formation of acidic metabolic by-products, helps to maintain the stable pH of the medium. It is suitable for cell culture in a non-CO₂ balanced environment. Leibovitz's L-15 medium is suitable for the culture of monkey kidney cells and HEP-2, the isolation of primary cells (such as embryonic and adult tissues), the culture of various viruses and the culture of neurons.
Leibovitz's L-15 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 300 mg/L L-Glutamine
• 10 mg/L Phenol red
• 900 mg/L D-galactose
• 550 mg/L Sodium pyruvate
• Phosphate buffer system Balanced salt
Without
• D-Glucose
• HEPES
• NaHCO₃
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: EUROPEAN JOURNAL OF MEDICAL RESEARCH (2023) IF: 4.2
DOI: 10.1186/s40001-023-01483-y
Product Cited: Penicillin-Streptomycin Solution, 100 ×, RPMI-1640 Medium, DMEM (High glucose) Medium, MCF 10A Cell Complete Medium, Leibovitz's L-15 Medium, DMEM/F12 Medium
Journal: ONCOLOGY REPORTS (2023) IF: 4.2
DOI: 10.3892/or.2023.8630
Product Cited: Leibovitz's L-15, Fetal Bovine Serum, DMEM/F12
Journal: CANCER BIOLOGY & THERAPY (2023) IF: 3.6
DOI: 10.1080/15384047.2023.2274120
Product Cited: hFOB 1.19 Cells Line, HOS Cells Line, Saos-2 Cells Line, SW 1353 Cells Line, Leibovitz's L-15, McCoy's 5A Complete Medium (10), MEM, DMEM/F12, Penicillin-Streptomycin Solution, 100×, Fetal Bovine Serum
Journal: IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2023) IF: 3.300
DOI: 10.1080/08923973.2023.2165943
Product Cited: MCF 10A Cells Line, Leibovitz's L-15, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×, MEM(with NEAA), MCF 10A Cells Complete Medium
Journal: DIGESTIVE DISEASES AND SCIENCES (2023) IF: 3.100
DOI: 10.1007/s10620-023-07936-y
Product Cited: SW948 Cells Line, Leibovitz's L-15