PM151010
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Product Introduction
Background
The buffer system of Leibovitz's L-15 medium is composed of phosphate and free amino acids, instead of the traditional sodium bicarbonate buffer system. Meanwhile, the substitution of galactose and sodium pyruvate for glucose prevents prevents the formation of acidic metabolic by-products, helps to maintain the stable pH of the medium. It is suitable for cell culture in a non-CO₂ balanced environment. Leibovitz's L-15 medium is suitable for the culture of monkey kidney cells and HEP-2, the isolation of primary cells (such as embryonic and adult tissues), the culture of various viruses and the culture of neurons.
Leibovitz's L-15 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 300 mg/L L-Glutamine
• 10 mg/L Phenol red
• 900 mg/L D-galactose
• 550 mg/L Sodium pyruvate
• Phosphate buffer system Balanced salt
Without
• D-Glucose
• HEPES
• NaHCO₃
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration | 1× |
Volume | 500mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Endotoxin Content | < 3 EU/mL |
pH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Green Features | Sustainable packaging |
Valid Period | 24 months |
Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
Shipping Condition | Room Temperature |
Publications
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY (2022) IF: 3.800
DOI: 10.1007/s11010-022-04525-x
Product Cited: LoVo Cells Line, SW620 Cells Line, SW480 [SW-480] Cells Line, HCT 116 Cells Line, Fetal Bovine Serum, Leibovitz's L-15, McCoy's 5A
Journal: EXPERIMENTAL CELL RESEARCH (2022) IF: 3.700
DOI: 10.1016/j.yexcr.2022.113035
Product Cited: SK-BR-3 [SKBR3] Cells Line, T-47D Cells Line, MDA-MB-231 Cells Line, MDA-MB-468 Cells Line, Leibovitz's L-15, McCoy's 5A
Journal: MOLECULAR BIOLOGY REPORTS (2022) IF: 2.700
DOI: 10.1007/s11033-022-08066-w
Product Cited: Leibovitz's L-15
Journal: Journal of Raman Spectroscopy (2022) IF: 2.500
DOI: 10.1002/jrs.6292
Product Cited: SW480 [SW-480] Cells Line, SW620 Cells Line, HCT 116 Cells Line, Leibovitz's L-15, McCoy's 5A
Journal: Reproductive Biology (2022) IF: 2.100
DOI: 10.1016/j.repbio.2022.100708
Product Cited: MDA-MB-231 Cells Line, BT-549 Cells Line, MCF 10A Cells Line, Leibovitz's L-15, RPMI-1640, DMEM(High glucose)