Leibovitz's L-15

Cat. No.: PM151010 Publications(90) Manual
Leibovitz's L-15 - 1
  • Leibovitz's L-15Leibovitz's L-15 - 1

PM151010

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Product Introduction

Background

The buffer system of Leibovitz's L-15 medium is composed of phosphate and free amino acids, instead of the traditional sodium bicarbonate buffer system. Meanwhile, the substitution of galactose and sodium pyruvate for glucose prevents prevents the formation of acidic metabolic by-products, helps to maintain the stable pH of the medium. It is suitable for cell culture in a non-CO₂ balanced environment. Leibovitz's L-15 medium is suitable for the culture of monkey kidney cells and HEP-2, the isolation of primary cells (such as embryonic and adult tissues), the culture of various viruses and the culture of neurons.
Leibovitz's L-15 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Ingredient Statement
With

• 300 mg/L L-Glutamine

• 10 mg/L Phenol red

• 900 mg/L D-galactose

• 550 mg/L Sodium pyruvate

• Phosphate buffer system Balanced salt

Without

• D-Glucose

• HEPES

• NaHCO₃

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

Specifications
Concentration
Volume 500mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.2-7.4
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Documents

Publications

Circ_0056618 enhances PRRG4 expression by competitively binding to miR-411-5p to promote the malignant progression of colorectal cancer

Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY (2022) IF: 3.800

DOI: 10.1007/s11010-022-04525-x

Product Cited: LoVo Cells Line, SW620 Cells Line, SW480 [SW-480] Cells Line, HCT 116 Cells Line, Fetal Bovine Serum, Leibovitz's L-15, McCoy's 5A

CKAP2L, transcriptionally inhibited by FOXP3, promotes breast carcinogenesis through the AKT/mTOR pathway

Journal: EXPERIMENTAL CELL RESEARCH (2022) IF: 3.700

DOI: 10.1016/j.yexcr.2022.113035

Product Cited: SK-BR-3 [SKBR3] Cells Line, T-47D Cells Line, MDA-MB-231 Cells Line, MDA-MB-468 Cells Line, Leibovitz's L-15, McCoy's 5A

Identification and characterization of the inhibitory effect of ART on colorectal cancer cells using Raman spectroscopy

Journal: Journal of Raman Spectroscopy (2022) IF: 2.500

DOI: 10.1002/jrs.6292

Product Cited: SW480 [SW-480] Cells Line, SW620 Cells Line, HCT 116 Cells Line, Leibovitz's L-15, McCoy's 5A

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