PM151010
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Product Introduction
Background
The buffer system of Leibovitz's L-15 medium is composed of phosphate and free amino acids, instead of the traditional sodium bicarbonate buffer system. Meanwhile, the substitution of galactose and sodium pyruvate for glucose prevents prevents the formation of acidic metabolic by-products, helps to maintain the stable pH of the medium. It is suitable for cell culture in a non-CO₂ balanced environment. Leibovitz's L-15 medium is suitable for the culture of monkey kidney cells and HEP-2, the isolation of primary cells (such as embryonic and adult tissues), the culture of various viruses and the culture of neurons.
Leibovitz's L-15 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 300 mg/L L-Glutamine
• 10 mg/L Phenol red
• 900 mg/L D-galactose
• 550 mg/L Sodium pyruvate
• Phosphate buffer system Balanced salt
Without
• D-Glucose
• HEPES
• NaHCO₃
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Publications
Journal: In Vitro Cellular & Developmental Biology-Animal (2021) IF: 2.700
DOI: 10.1007/s11626-021-00603-4
Product Cited: SW480 [SW-480] Cells Line, SW620 Cells Line, HCT 116 Cells Line, LoVo Cells Line, Caco-2 Cells Line, Leibovitz's L-15, Ham's F-12K, McCoy's 5A
Journal: Journal of Experimental & Clinical Cancer Research (2021) IF: 11.300
DOI: 10.1186/s13046-021-01894-x
Product Cited: RPMI-1640, DMEM(High glucose), Leibovitz's L-15, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×
Journal: Aquaculture (2020) IF: 4.500
DOI: 10.1016/j.aquaculture.2020.735115
Product Cited: Leibovitz's L-15
Journal: OncoTargets and Therapy (2020) IF: 4.000
DOI: 10.2147/OTT.S281962
Product Cited: Leibovitz's L-15 Medium, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100 ×, MEM Medium, DMEM/F12 Medium, McCoy's 5A Medium, Leibovitz's L-15, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×, MEM(with NEAA), 10 mg/mL Recombinant Human Insulin Solution, McCoy's 5A, DMEM/F12
Journal: Marine Biotechnology (2019) IF: 3.000
DOI: 10.1007/s10126-019-09902-0
Product Cited: Leibovitz's L-15
