Leibovitz's L-15

Cat. No.: PM151010 Publications(90) Manual
Leibovitz's L-15 - 1
  • Leibovitz's L-15Leibovitz's L-15 - 1

PM151010

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Product Introduction

Background

The buffer system of Leibovitz's L-15 medium is composed of phosphate and free amino acids, instead of the traditional sodium bicarbonate buffer system. Meanwhile, the substitution of galactose and sodium pyruvate for glucose prevents prevents the formation of acidic metabolic by-products, helps to maintain the stable pH of the medium. It is suitable for cell culture in a non-CO₂ balanced environment. Leibovitz's L-15 medium is suitable for the culture of monkey kidney cells and HEP-2, the isolation of primary cells (such as embryonic and adult tissues), the culture of various viruses and the culture of neurons.
Leibovitz's L-15 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Ingredient Statement
With

• 300 mg/L L-Glutamine

• 10 mg/L Phenol red

• 900 mg/L D-galactose

• 550 mg/L Sodium pyruvate

• Phosphate buffer system Balanced salt

Without

• D-Glucose

• HEPES

• NaHCO₃

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

Specifications
Concentration
Volume 500mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.2-7.4
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Documents

Publications

Zinc finger protein 468 up-regulation of TFAM contributes to the malignant growth and cisplatin resistance of breast cancer cells

Journal: Cell Division (2024) IF: 2.3

DOI: 10.1186/s13008-024-00113-1

Product Cited: MCF7 [MCF-7] Cells Line, MDA-MB-231 Cells Line, MDA-MB-468 Cells Line, DMEM/F12, MEM(with NEAA), Leibovitz's L-15, Penicillin-Streptomycin Solution, 100×, Annexin V-APC/PI Apoptosis Kit

Circ_0049271 targets the miR-1197/PTRF axis to attenuate the malignancy of osteosarcoma

Journal: Cancer Biomarkers (2024) IF: 2.2

DOI: 10.3233/CBM-230191

Product Cited: McCoy's 5A Medium, HOS Cells Line, Leibovitz's L-15 Medium, Fetal Bovine Serum, hFOB 1.19 Cells Line, DMEM (High glucose) Medium, Saos-2 Cells Line

CAPRIN1 Transcriptionally Activated PLPP4 to Inhibit DOX Sensitivity and Promote Breast Cancer Progression

Journal: CELL BIOCHEMISTRY AND BIOPHYSICS (2024) IF: 1.8

DOI: 10.1007/s12013-024-01614-0

Product Cited: Fetal Bovine Serum, Donor Equine Serum, MCF 10A Cells Line, MDA-MB-231 Cells Line, Leibovitz's L-15 Medium, HCC1937 Cells Line, 293T [HEK-293T] Cells Line, RPMI-1640 Medium, DMEM (High glucose) Medium, DMEM/F12 Medium

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